Lissa1: August6-August14: Difference between revisions

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#PCR amplify the pGEV band I purified yesterday -DONE
#PCR amplify the pGEV band I purified yesterday -DONE
#PCR out GEV insert from the original pGEV DNA -DONE
#PCR out GEV insert from the original pGEV DNA -DONE
#Run gels of these PCRs and do gel extraction
#Run gels of these PCRs and do gel extraction -DONE
#Digest pGEV
#Digest pGEV - no time
#Setup overnight of pRS-405 in DH5alpha, in LB + Amp
#Setup overnight of pRS-405 in DH5alpha, in LB + Amp -DONE
#Streak new plate -DONE
#Setup overnight of samples for Fus3, etc -DONE
#Setup overnight of samples for Fus3, etc -DONE



Revision as of 06:17, 10 August 2006

August 6

August 7

  1. Pour new SUMO gel -DONE
  2. Pour new small gels -DONE
  3. Set up overnights for the following experiments:
    1. Nocodazole arrest -DONE
    2. Induction experiment -DONE
    3. Make more unarrested 403 for phosphatase Western -DONE
  4. Make media for the nocodazole arrest. -DONE
  5. Plan EVERYTHING -CHECK
    1. Cloning
    2. Finalized experiments
    3. What to do about luminol/ECF deal? - OOPS, stil don't know
  6. Integrate new-found numbers/data in to model -DONE
    1. Maybe update model to include Michaelis-Menton kinetics -NOT YET, talk to Ty

August 8

  1. Do nocodazole arrest! -CELLS WON'T GROW:(
  2. Do induction experiment! -DONE
  3. Set up overnights for the following experiments: -MOVED UNTIL TOMORROW
    1. Fus3/Active Fus3 gels
    2. Phosphatase control
  4. PCR up pGEV out of ACLY700 -DONE
  5. Run a gel of the PCR -DONE
  6. If there's time, run a second gel and extract and purify the pGEV (which might not be pGEV, based on the sequencing data) -DONE

August 9

  1. PCR amplify the pGEV band I purified yesterday -DONE
  2. PCR out GEV insert from the original pGEV DNA -DONE
  3. Run gels of these PCRs and do gel extraction -DONE
  4. Digest pGEV - no time
  5. Setup overnight of pRS-405 in DH5alpha, in LB + Amp -DONE
  6. Streak new plate -DONE
  7. Setup overnight of samples for Fus3, etc -DONE
  • Unfortunately, I've got a cold and need to rest:(

August 10

  1. Long incubation, wash, image Fus3 gel.

August 11

  1. Cut SUMO gel, set up transfer.

August 12

  1. Wash and image SUMO gel.