Lissa1: August6-August14: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 49: | Line 49: | ||
==August 11== | ==August 11== | ||
#Make new SCR-u-h media! | #Make new SCR-u-h media! | ||
#Do native extraction and phosphatase experiment on Noc. arrested yeast | |||
#Load SUMO gel and start run | |||
#PCR up GEV insert out of miniprepped pGEV DNA | |||
==August 12== | ==August 12== | ||
#Wash and image SUMO gel. | #Wash and image SUMO gel. |
Revision as of 06:21, 11 August 2006
August 6
August 7
- Pour new SUMO gel -DONE
- Pour new small gels -DONE
- Set up overnights for the following experiments:
- Nocodazole arrest -DONE
- Induction experiment -DONE
- Make more unarrested 403 for phosphatase Western -DONE
- Make media for the nocodazole arrest. -DONE
- Plan EVERYTHING -CHECK
- Cloning
- Finalized experiments
- What to do about luminol/ECF deal? - OOPS, stil don't know
- Integrate new-found numbers/data in to model -DONE
- Maybe update model to include Michaelis-Menton kinetics -NOT YET, talk to Ty
August 8
- Do nocodazole arrest! -CELLS WON'T GROW:(
- Do induction experiment! -DONE
- Set up overnights for the following experiments: -MOVED UNTIL TOMORROW
- Fus3/Active Fus3 gels
- Phosphatase control
- PCR up pGEV out of ACLY700 -DONE
- Run a gel of the PCR -DONE
- If there's time, run a second gel and extract and purify the pGEV (which might not be pGEV, based on the sequencing data) -DONE
August 9
- PCR amplify the pGEV band I purified yesterday -DONE
- PCR out GEV insert from the original pGEV DNA -DONE
- Run gels of these PCRs and do gel extraction -DONE
- Digest pGEV - no time
- Setup overnight of pRS-405 in DH5alpha, in LB + Amp -DONE
- Streak new plate -DONE
- Setup overnight of samples for Fus3, etc -DONE
- Unfortunately, I've got a cold and need to rest:(
August 10
- Prep Fus3 samples (all the way to sample buffer) and store -DOne
- Get concentrations on all DNA - DONE
- Miniprep 405 out of E. coli -DONE
- Streak out new 403 plate from freezer stock -DONE
- Digest pGEV, run on gel to check -DONE
- Design diagnostics for prs405 DNA -DONE
- Digest 405 -DONE
- Do PCR cleanup - DONE
August 11
- Make new SCR-u-h media!
- Do native extraction and phosphatase experiment on Noc. arrested yeast
- Load SUMO gel and start run
- PCR up GEV insert out of miniprepped pGEV DNA
August 12
- Wash and image SUMO gel.