Lissa1: June14-June21

Wednesday, June 14

1. Grow up a culture of pGEV.
2. SUMO
   1. Pour gel
      1. Fill stacking up to top
      2. Insert comb at angle
      3. If at first it doesn't succeed...
      4. More TEMED in running?
   2. Run gel
      1. Voltage = 500 (Note: load sample in center wells to mitigate smiling)
      2. Will it make a difference if we refresh buffer over time?
3. Gal4 question?

Thursday, June 15

1. Miniprep of pGEV
2. Digest of pGEV.
   1. enzyme SnaBi?
3. Overnight of yeast.
4. Make plates?
5. Check on SUMO gel?

Friday, June 16

1. Yeast-pGEV Transformation
2. Start Western. Overnight incubation w. antibody.

Weekend, June 17-18

1. Saturday
   1. Wash and image.

Monday, June 19

1. Take out transformations
2. PCR verification
3. Sequencing