LncRNA ToDoList: Difference between revisions
Huiming Ding (talk | contribs) No edit summary |
Huiming Ding (talk | contribs) No edit summary |
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==March 7, 2012== | |||
lncRNA regulation mechanism | |||
CIS model | |||
Trans model | |||
lncRNA expression | |||
median normalized - DESeq | |||
Conserved lncRNA in mouse and human for further knock down | |||
Paul - H2AZ | |||
active - H3K4mer3 | |||
silent - H3K27mer3 | |||
poised - both H3K4mer3 and H3K27mer3, bivalent | |||
==February 23, 2012== | ==February 23, 2012== | ||
Joe | Joe |
Revision as of 09:08, 7 March 2012
March 7, 2012
lncRNA regulation mechanism
CIS model
Trans model
lncRNA expression median normalized - DESeq
Conserved lncRNA in mouse and human for further knock down
Paul - H2AZ active - H3K4mer3 silent - H3K27mer3 poised - both H3K4mer3 and H3K27mer3, bivalent
February 23, 2012
Joe
set up a database and a wiki
lncrna analysis with reduced cutoff at 0.5 from 1
transcription factor & the dip of the histone modification
February 8,2012
h2az
divide genes into different categories such as lncrnas , mirrnas , and some specific processes/pathways,
February 3, 2012
LncRNAs, their temproal nd spatial function in the regulation of expression.
1. There is a gap between day 3 and day 6, may add day 4 and 5 rna-seq data
2. There possibly a transition during day 1 and day 3 that lunch renascence and mirrnas are associated in early stage of differentiation
3. The network makes sense to the stage specific differentiation. However, add some mirrnasback to the network ?
4. Mesp4 is picked for downstreame study because mesp4 seems to be a master regulator in early stage at the starting point. There is a linear regulation leading to the carjacking differentiation.
5.does mesp1 overexpfession rescue the phenotype?
6. Does mesp1 knock outhave the samephenotype?
7. How lncrna affects expression?