Loading dye

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==Loading dye==
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==Purpose==
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Loading dye is mix with DNA samples for use in agarose gel electrophoresis.  It generally contains a dye to assess how "fast" your gel is running and a reagent to render your samples denser than the running buffer (so that the samples sink in the well).
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==Procurement==
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Here's a sample recipe for loading dye.
*Ficoll 400 (in 68-558F)
*Ficoll 400 (in 68-558F)
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Dissolve 1.5 g of Ficoll in 10 mL of deionized water.  Add very small amounts of Orange G dye such that the loading dye is dark orange.  Store in small aliquots at 4°C (room temperature is okay too). To use, add and mix 1/5th volume of loading dye to DNA solutions prior to loading into the wells of gels.
Dissolve 1.5 g of Ficoll in 10 mL of deionized water.  Add very small amounts of Orange G dye such that the loading dye is dark orange.  Store in small aliquots at 4°C (room temperature is okay too). To use, add and mix 1/5th volume of loading dye to DNA solutions prior to loading into the wells of gels.
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==Other dyes==
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===Specific recipes===
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*[[Knight:Loading dye]]
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==Use==
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*Orange G: generally runs very fast (<100 bp)
*Bromophenol blue: purple, generally runs at ~500bp (depending on percentage agarose)
*Bromophenol blue: purple, generally runs at ~500bp (depending on percentage agarose)
*Xylene cyanol: blue, runs at ~4kb
*Xylene cyanol: blue, runs at ~4kb

Revision as of 11:53, 25 August 2006

Contents

Purpose

Loading dye is mix with DNA samples for use in agarose gel electrophoresis. It generally contains a dye to assess how "fast" your gel is running and a reagent to render your samples denser than the running buffer (so that the samples sink in the well).

Procurement

Here's a sample recipe for loading dye.

  • Ficoll 400 (in 68-558F)
  • Deionized water
  • Orange G dye

Dissolve 1.5 g of Ficoll in 10 mL of deionized water. Add very small amounts of Orange G dye such that the loading dye is dark orange. Store in small aliquots at 4°C (room temperature is okay too). To use, add and mix 1/5th volume of loading dye to DNA solutions prior to loading into the wells of gels.

Specific recipes

Use

  • Orange G: generally runs very fast (<100 bp)
  • Bromophenol blue: purple, generally runs at ~500bp (depending on percentage agarose)
  • Xylene cyanol: blue, runs at ~4kb
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