Loading dye: Difference between revisions
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==Loading dye== | ==Purpose== | ||
Loading dye is mix with DNA samples for use in agarose gel electrophoresis. It generally contains a dye to assess how "fast" your gel is running and a reagent to render your samples denser than the running buffer (so that the samples sink in the well). | |||
==Procurement== | |||
Here's a sample recipe for loading dye. | |||
*Ficoll 400 (in 68-558F) | *Ficoll 400 (in 68-558F) | ||
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Dissolve 1.5 g of Ficoll in 10 mL of deionized water. Add very small amounts of Orange G dye such that the loading dye is dark orange. Store in small aliquots at 4°C (room temperature is okay too). To use, add and mix 1/5th volume of loading dye to DNA solutions prior to loading into the wells of gels. | Dissolve 1.5 g of Ficoll in 10 mL of deionized water. Add very small amounts of Orange G dye such that the loading dye is dark orange. Store in small aliquots at 4°C (room temperature is okay too). To use, add and mix 1/5th volume of loading dye to DNA solutions prior to loading into the wells of gels. | ||
== | ===Specific recipes=== | ||
*[[Knight:Loading dye]] | |||
==Use== | |||
*Orange G: generally runs very fast (<100 bp) | |||
*Bromophenol blue: purple, generally runs at ~500bp (depending on percentage agarose) | *Bromophenol blue: purple, generally runs at ~500bp (depending on percentage agarose) | ||
*Xylene cyanol: blue, runs at ~4kb | *Xylene cyanol: blue, runs at ~4kb | ||
Revision as of 08:53, 25 August 2006
Purpose
Loading dye is mix with DNA samples for use in agarose gel electrophoresis. It generally contains a dye to assess how "fast" your gel is running and a reagent to render your samples denser than the running buffer (so that the samples sink in the well).
Procurement
Here's a sample recipe for loading dye.
- Ficoll 400 (in 68-558F)
- Deionized water
- Orange G dye
Dissolve 1.5 g of Ficoll in 10 mL of deionized water. Add very small amounts of Orange G dye such that the loading dye is dark orange. Store in small aliquots at 4°C (room temperature is okay too). To use, add and mix 1/5th volume of loading dye to DNA solutions prior to loading into the wells of gels.
Specific recipes
Use
- Orange G: generally runs very fast (<100 bp)
- Bromophenol blue: purple, generally runs at ~500bp (depending on percentage agarose)
- Xylene cyanol: blue, runs at ~4kb
