Loading dye: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
 
(2 intermediate revisions by 2 users not shown)
Line 1: Line 1:
==Loading dye==
==Purpose==
Loading dye is mixed with samples for use in gel electrophoresis.  It generally contains a dye to assess how "fast" your gel is running and a reagent to render your samples denser than the running buffer (so that the samples sink in the well).


*Ficoll 400 (in 68-558F)
==Types of loading dyes==
*Deionized water
*Orange G dye


Dissolve 1.5 g of Ficoll in 10 mL of deionized water.  Add very small amounts of Orange G dye such that the loading dye is dark orange.  Store in small aliquots at 4°C (room temperature is okay too). To use, add and mix 1/5th volume of loading dye to DNA solutions prior to loading into the wells of gels.
[[Agarose gel loading dye]]
 
==Other dyes==
*Bromophenol blue: purple, generally runs at ~500bp (depending on percentage agarose)
*Xylene cyanol: blue, runs at ~4kb

Latest revision as of 12:53, 25 August 2006

Purpose

Loading dye is mixed with samples for use in gel electrophoresis. It generally contains a dye to assess how "fast" your gel is running and a reagent to render your samples denser than the running buffer (so that the samples sink in the well).

Types of loading dyes

Agarose gel loading dye

Retrieved from "https://openwetware.org/mediawiki/index.php?title=Loading_dye&oldid=64697"