Luckau Protocols:1Kb Ladder

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Ladder Mix

[edit]Manufacturer specifications 0.1µg ladder / mm width of well STK at 1.0µg/µL Media:Invitrogen_1KbLadderProductManual.pdf [edit]Other Knowns well width (24-tooth comb) = 6mm Jeremy (Bohonak lab) uses 5µL PCR product + 1.5µL dye [edit]Calculations 6mm x (0.1µg ladder)/(1mm width) x (1µL/1µg) = 0.6µL ladder per well Per well: 0.6µL ladder + 4.4µL H2O + 1.5µL loading dye = 6.5µL total ladder mix (loading dye = xylene cyanol and bromophenol, made by Bohonak lab) [edit]Make Ladder Mix 120µL ladder + 880µL H2O + 300µL loading dye = 1300µL ladder mix

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