Luckau Protocols:FragmentAnalysisSubmission
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Fragment Analysis Submission |
Purpose
This protocol outlines how to submit samples for fragment analysis at the University of Arizona Genetics Core (samples are light-sensitive PCR product).
Protocol
Submit Order Online
- Log-in to services.arl.arizona.edu
- Click "Create Submission" (at bottom of page)
- - Click "Fragment Analysis / STR Typing"
- - Click Account Number "111111"
- In the "Grid Map" panel, click "Paste Sample Map"
- - copy sample grid from Excel spreadsheet
- use only alpha numeric characters as well as "-" and "_" for special characters
- sample names must be unique
- - copy sample grid from Excel spreadsheet
- In the "Fragment Grid" panel:
- - Dye Set:
- G5 (5-dye) - UAGC will load ladder into the orange (ROX) color lane
- D (4-dye) - UAGC will load ladder into the red (PET) color lane
- - Dilution Ratio:
- 1/20 - this is standard; you shouldn't have to alter this
- - Dye Set:
- Click "Submit Grid"
- Log the four-letter submission ID
- "Submission: frg-YY-MM-DD-XXXX"
Ship Order
- write the four-letter submission ID onto your plate
- this is how the lab technicians match the online order to the samples mailed
- wrap plate in old FedEx bubble mailers to protect plate from light and the inherent bashing around that happens in the shipping process
- follow shipping protocol