Luckau Protocols:MgCl2: Difference between revisions
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* If this 500 mM MgCl<sub>2</sub> is being used as stock solution for | * If this 500 mM MgCl<sub>2</sub> is being used as the stock solution for PCR Buffers A-H, then leave in the 250 mL glass media bottle and store at room temperature on the chemical shelf. | ||
Revision as of 16:52, 26 September 2012
| MgCl2 Protocol |
Purpose
In the Clark Lab, 500 mM MgCl2 is used as a stock solution for making the PCR Buffer series (A through H)
Info
- Magnesium chloride, MgCl2 (anhydrous)
- we currently use Acros Organics, Product number 223211000, Lot A0316391
- MW = 95.21 g/mol
To make 150 mL of 500 mM MgCl2
Calculations
- [math]\displaystyle{ 150 \mathrm{mL} \bullet \tfrac{500 \mathrm{mmol}}{\mathrm{L}}\ \bullet \tfrac{\mathrm{L}}{1000 \mathrm{mL}}\ \bullet \tfrac{95.21 \mathrm{g}}{\mathrm{mol}}\ = 7.14075 \mathrm{g} }[/math]
Protocol
- Using digital scale, place 7.14 g MgCl2 powder into weigh boat
- In 250 mL glass media bottle, mix 7.14 g MgCl2 with approximately 100 mL H2O
- Place bottle on magnetic stirrer with stirbar until dissolved (note: the dissolving of MgCl2 with H2O is an exothermic reaction - you may notice the solution fizz a bit when adding water to the powder. Just don't stand over it and inhale, as a general rule)
- Add H2O to bring volume to 150 mL
- If this 500 mM MgCl2 is being used as the stock solution for PCR Buffers A-H, then leave in the 250 mL glass media bottle and store at room temperature on the chemical shelf.
