Luckau Protocols:PCR: Difference between revisions
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| width="600" style="background-color: #BCED91;" align="center"| <span style="font-size:32px;"> Agarose Gel Protocol </span> | |||
|align="left" style="background-color: #9DB68C;" | | |||
<span style="font-size:16px;"> [[User:Tara_K._Luckau | Tara K. Luckau's Home Page]] </span> | |||
[[User:Tara_K._Luckau/Notebook/Team_ConGen | Conservation Genetics Lab Notebook]] | |||
[[Luckau_Protocols | Tara's Protocols]] | |||
* [[Luckau_Protocols:NanoDrop | NanoDrop]] | |||
* [[Luckau_Protocols:PCR | PCR]] | |||
* [[Luckau_Protocols:Agarose_Gel | Agarose Gel]] | |||
* [[Luckau_Protocols:Low_TE | Low TE]] | |||
* [[Luckau_Protocols:Tris-Cl | Tris-Cl]] | |||
* [[Luckau_Protocols:KCl | KCl]] | |||
|} | |||
==Purpose== | |||
PCR, polymerase chain reaction, is used to amplify target fragments of DNA for analysis. | |||
===Reagents=== | |||
* template DNA (sample) | |||
* dNTPs | |||
* Taq polymerase | |||
* Buffer (MgCl<sub>2</sub>, KCl) | |||
* primers | |||
===Consumables=== | |||
* PCR plate | |||
* pipet and tips | |||
==Protocol== | |||
===Preparation=== | |||
# fill ice bucket | |||
# wipe work area down with 10% bleach | |||
===Master Mix=== | |||
# According to the table above, mix agarose powder and 1x TAE in Erlenmeyer flask; swirl | |||
Revision as of 21:02, 9 February 2011
| Agarose Gel Protocol |
Purpose
PCR, polymerase chain reaction, is used to amplify target fragments of DNA for analysis.
Reagents
- template DNA (sample)
- dNTPs
- Taq polymerase
- Buffer (MgCl2, KCl)
- primers
Consumables
- PCR plate
- pipet and tips
Protocol
Preparation
- fill ice bucket
- wipe work area down with 10% bleach
Master Mix
- According to the table above, mix agarose powder and 1x TAE in Erlenmeyer flask; swirl
