Luckau Protocols:ShipDryIce: Difference between revisions
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===Packaging=== | ===Packaging=== | ||
[[Image:20120731_FedEx.png|900 px]] | * to send samples to UAGC for fragment analysis (genotyping), fill out forms as follows: | ||
[[Image:Class9_DryIce_TKL.jpg|600 px]] | : (note Account Number and Internal Billing Reference! very important!) | ||
* [[Image:20120731_FedEx.png|900 px]] | |||
* [[Image:Class9_DryIce_TKL.jpg|600 px]] | |||
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* [http://www.dryicesource.com/dryice/dryicesafety.php Continental Carbonic Dry Ice Safety] | * [http://www.dryicesource.com/dryice/dryicesafety.php Continental Carbonic Dry Ice Safety] | ||
* [http://www.dryicesource.com/pdf/SafetyEnglish.pdf Continental Carbonic Dry Ice Safety Brochure] | * [http://www.dryicesource.com/pdf/SafetyEnglish.pdf Continental Carbonic Dry Ice Safety Brochure] | ||
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* Do '''NOT''' place dry ice in any sealed container (gas is less dense than the solid, so sublimation causes the CO<sub>2</sub> to expand) | * Do '''NOT''' place dry ice in any sealed container (gas is less dense than the solid, so sublimation causes the CO<sub>2</sub> to expand) | ||
:* '''DO''' use dry ice in a ventilated location, and store in an insulated container | :* '''DO''' use dry ice in a ventilated location, and store in an insulated container | ||
Revision as of 15:02, 2 August 2012
| Shipping Dry Ice |
Purpose
When shipping genomic DNA or PCR product, it is helpful (and possibly necessary) to keep the samples frozen. Dry ice (solid CO2) is the preferred cooling agent for mailing shipments.
Benefites of dry ice:
- sublimation of solid CO2 means there's no messy liquid state, as with 'regular' ice
- dry ice keeps your samples colder for longer (dry ice is -78°C) (household freezers are typically -20°C)
This protocol gives details for shipping samples out for fragment analysis (samples are light-sensitive PCR product).
Protocol
Materials Needed
- From Department of Biology office (NLS 101)
- US Domestic Airbill
- Dry ice label (class 9, UN 1845)
- resealable adhesive plastic pouch
- US Domestic Airbill
- Shipping container
- insulated container (styrofoam) with cardboard outer casing
- dry ice
- available from common room 320 (dry ice is re-stocked each Tuesday and Friday)
- remember to sign-out how much you're taking
Packaging
- to send samples to UAGC for fragment analysis (genotyping), fill out forms as follows:
- (note Account Number and Internal Billing Reference! very important!)
Additional Resources
- Amherst College Dry Ice Shipping
- Continental Carbonic Dry Ice Shipping
- Continental Carbonic Dry Ice Safety
- Continental Carbonic Dry Ice Safety Brochure
Safety
- Do NOT touch dry ice (at -78°C, it can 'burn' your skin)
- DO handle dry ice using protective gloves
- Do NOT place dry ice in any sealed container (gas is less dense than the solid, so sublimation causes the CO2 to expand)
- DO use dry ice in a ventilated location, and store in an insulated container
