Luckau Protocols:TAE: Difference between revisions
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==Protocol== | ==Protocol== | ||
===To make | ===To make 500mL of 50x TAE=== | ||
(2M Tris, 2M acetic acid, 50mM EDTA): | (2M Tris, 2M acetic acid, 50mM EDTA): | ||
* dissolve | * dissolve 121 g Tris-base (FW - 121.14) in about 300 mL MilliQ H<sub>2</sub>O | ||
* add | * add 28.55 mL glacial acetic acid | ||
* add | * add 50 mL 0.5M EDTA (pH 8.0) | ||
* add MilliQ H<sub>2</sub>O to bring total volume to | * add MilliQ H<sub>2</sub>O to bring total volume to 500mL | ||
Do not autoclave (I don't know why) | Do not autoclave (I don't know why) | ||
Revision as of 11:00, 8 June 2011
| TAE |
Purpose
TAE is a commonly used buffer for making and running DNA agarose gels. It is cheap to make, easy to store, and buffers well under higher running voltages.
It is convenient to make a stock solution of 50x TAE for easy storage.
Protocol
To make 500mL of 50x TAE
(2M Tris, 2M acetic acid, 50mM EDTA):
- dissolve 121 g Tris-base (FW - 121.14) in about 300 mL MilliQ H2O
- add 28.55 mL glacial acetic acid
- add 50 mL 0.5M EDTA (pH 8.0)
- add MilliQ H2O to bring total volume to 500mL
Do not autoclave (I don't know why)
To make 1L of 1x TAE from 50x TAE
- 20mL 50x TAE + 980 NanoPure H2O
Sources
- OpenWetWare's Materials TAE page
- OpenWetWare's User, Silver Lab TAE
- About.com's Biotech page
