Luckau Protocols:Tris-Cl: Difference between revisions
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===0.5M Tris-Cl, pH 8.0=== | ===0.5M Tris-Cl, pH 8.0=== | ||
<math>500 mL \bullet \frac{0.5 mol}{L}\ \bullet \frac{L}{1000 mL}\ \bullet \frac{157.56 g}{mol}\ = 39.39 g</math> | * <math>500 mL \bullet \frac{0.5 mol}{L}\ \bullet \frac{L}{1000 mL}\ \bullet \frac{157.56 g}{mol}\ = 39.39 g</math> | ||
'''to make \tfrac{1}{2} liter of 0.5M Tris-Cl, pH 8.0:''' | * '''to make \tfrac{1}{2} liter of 0.5M Tris-Cl, pH 8.0:''' | ||
# In 1 L beaker, mix 39.39 g Tris-Cl in 400 mL H<sub>2</sub>O | # In 1 L beaker, mix 39.39 g Tris-Cl in 400 mL H<sub>2</sub>O | ||
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===1M Tris-Cl, pH 8.0=== | ===1M Tris-Cl, pH 8.0=== | ||
<math>500 mL \bullet \frac{1 mol}{L}\ \bullet \frac{L}{1000 mL}\ \bullet \frac{157.56 g}{mol}\ = 78.78 g</math> | * <math>500 mL \bullet \frac{1 mol}{L}\ \bullet \frac{L}{1000 mL}\ \bullet \frac{157.56 g}{mol}\ = 78.78 g</math> | ||
'''to make \tfrac{1}{2} liter of 1M Tris-Cl, pH 8.0:''' | * '''to make \tfrac{1}{2} liter of 1M Tris-Cl, pH 8.0:''' | ||
# In 1 L beaker, mix 78.78 g Tris-Cl in 400 mL H<sub>2</sub>O | # In 1 L beaker, mix 78.78 g Tris-Cl in 400 mL H<sub>2</sub>O | ||
Revision as of 13:31, 22 November 2010
Back to User:Tara_K._Luckau
Back to Luckau Protocols
Purpose
- Tris-Cl is a buffering agent (made from Tris base and HCl)
- We use it as a stock solution for Low TE
Info
- tris [hydroxymethyl] amino methane (C4H11NO3, 2-Amino-2-hydroxymethyl-propane-1,3-diol)
- MW = 157.56 g/mol (MW = molecular weight, same as formula weight, FW)
Protocol
0.5M Tris-Cl, pH 8.0
- [math]\displaystyle{ 500 mL \bullet \frac{0.5 mol}{L}\ \bullet \frac{L}{1000 mL}\ \bullet \frac{157.56 g}{mol}\ = 39.39 g }[/math]
- to make \tfrac{1}{2} liter of 0.5M Tris-Cl, pH 8.0:
- In 1 L beaker, mix 39.39 g Tris-Cl in 400 mL H2O
- Put beaker on hotplate with stirbar until dissolved
- Using concentrated aqueous HCl to adjust to pH 8.0
- Add H2O to bring volume to 500 mL
- Autoclave solution, store at room temperature
1M Tris-Cl, pH 8.0
- [math]\displaystyle{ 500 mL \bullet \frac{1 mol}{L}\ \bullet \frac{L}{1000 mL}\ \bullet \frac{157.56 g}{mol}\ = 78.78 g }[/math]
- to make \tfrac{1}{2} liter of 1M Tris-Cl, pH 8.0:
- In 1 L beaker, mix 78.78 g Tris-Cl in 400 mL H2O
- Put beaker on hotplate with stirbar until dissolved
- Using concentrated aqueous HCl to adjust to pH 8.0
- Add H2O to bring volume to 500 mL
- Autoclave solution, store at room temperature
