Luckau Protocols:Tris-Cl

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Purpose

• Tris-Cl is a buffering agent (made from Tris base and HCl)
• We use it as a stock solution for Low TE

Info

• tris [hydroxymethyl] amino methane (C₄H₁₁NO₃, 2-Amino-2-hydroxymethyl-propane-1,3-diol)
• MW = 157.56 g/mol (MW = molecular weight, same as formula weight, FW)

bring to desired pH with concentrated HClaq

Protocol

0.5M Tris-Cl, pH 8.0

[math]\displaystyle{ 500 mL \bullet \frac{0.5 mol}{L}\ \bullet \frac{L}{1000 mL}\ \bullet \frac{157.56 g}{mol}\ = 39.39 g }[/math]

to make \tfrac{1}{2} liter of 0.5M Tris-Cl, pH 8.0:

1. In 1 L beaker, mix 39.39 g Tris-Cl in 400 mL H₂O
2. Put beaker on hotplate with stirbar until dissolved
3. Using concentrated aqueous HCl to adjust to pH 8.0
4. Add H₂O to bring volume to 500 mL
5. Autoclave solution, store at room temperature

1M Tris-Cl, pH 8.0

[math]\displaystyle{ 500 mL \bullet \frac{1 mol}{L}\ \bullet \frac{L}{1000 mL}\ \bullet \frac{157.56 g}{mol}\ = 78.78 g }[/math]

to make \tfrac{1}{2} liter of 1M Tris-Cl, pH 8.0:

1. In 1 L beaker, mix 78.78 g Tris-Cl in 400 mL H₂O
2. Put beaker on hotplate with stirbar until dissolved
3. Using concentrated aqueous HCl to adjust to pH 8.0
4. Add H₂O to bring volume to 500 mL
5. Autoclave solution, store at room temperature