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(New page: ==Procedures== ===Quantification=== # '''Instument''' Qubit Fluoromete # '''Setting''' Broad Range #* Use Broad Range Standards in BR kit # Turn lights off, reagents are light sensitive #...)
Current revision (16:08, 5 November 2010) (view source)
 
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==Procedures==
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==Research interests==
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===Quantification===
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# '''Instument''' Qubit Fluoromete
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* [[Lum:Microbial Community Analysis| Microbial Community Analysis]]: Bacterial and Fungal Diversity Associated with the Rhizosphere of the nitrogen fixing plant ''Lupines chamissonis''
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# '''Setting''' Broad Range
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* [[Lum:Tommy|Tommy]]
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#* Use Broad Range Standards in BR kit
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* [[Lum:Jodie|Jodie]]
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# Turn lights off, reagents are light sensitive
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* [[Lum:Elise|Elise]]
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# Prepare '''Working Solution'''
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* [[Lum:Amanda|Amanda]]
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#* 199 µl '''Quant iT Buffer''' : 1 µl '''Quant iT Reagent'''
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* [[Lum:Michael P.|Michael P.]]
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#*199:1 for every sample needed (ex.10 samples will be 2000 µl total)
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# Prepare Samples
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# *190 µl working solution + 10 µl standard 1 solution
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# *190 µl working solution + 10 µl standard 2 solution
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# *195 µl working solution + 5 µl of DNA
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# Vortex for 2-3 minutes
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# Incubate 15 minutes
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# Follow On Screen Directions
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