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Michelle Lum (Talk | contribs)
(New page: ==Procedures== ===Quantification=== # '''Instument''' Qubit Fluoromete # '''Setting''' Broad Range #* Use Broad Range Standards in BR kit # Turn lights off, reagents are light sensitive #...)
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Revision as of 23:51, 13 September 2010

Procedures

Quantification

  1. Instument Qubit Fluoromete
  2. Setting Broad Range
    • Use Broad Range Standards in BR kit
  3. Turn lights off, reagents are light sensitive
  4. Prepare Working Solution
    • 199 µl Quant iT Buffer : 1 µl Quant iT Reagent
    • 199:1 for every sample needed (ex.10 samples will be 2000 µl total)
  5. Prepare Samples
  6. *190 µl working solution + 10 µl standard 1 solution
  7. *190 µl working solution + 10 µl standard 2 solution
  8. *195 µl working solution + 5 µl of DNA
  9. Vortex for 2-3 minutes
  10. Incubate 15 minutes
  11. Follow On Screen Directions
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