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(New page: ==Procedures== ===Quantification=== # '''Instument''' Qubit Fluoromete # '''Setting''' Broad Range #* Use Broad Range Standards in BR kit # Turn lights off, reagents are light sensitive #...)
(Procedures)
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==Procedures==
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==Research interests==
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===Quantification===
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# '''Instument''' Qubit Fluoromete
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# [Microbial Community Analysis]: Bacterial and Fungal Diversity Associated with the Rhizosphere of the nitrogen fixing plant “lupines chamissonis”
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# '''Setting''' Broad Range
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# [Tommy]
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#* Use Broad Range Standards in BR kit
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# [Jodie]
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# Turn lights off, reagents are light sensitive
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# [Elise]
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# Prepare '''Working Solution'''
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# [Amanda]
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#* 199 µl '''Quant iT Buffer''' : 1 µl '''Quant iT Reagent'''
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#*199:1 for every sample needed (ex.10 samples will be 2000 µl total)
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# Prepare Samples
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# *190 µl working solution + 10 µl standard 1 solution
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# *190 µl working solution + 10 µl standard 2 solution
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# *195 µl working solution + 5 µl of DNA
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# Vortex for 2-3 minutes
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# Incubate 15 minutes
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# Follow On Screen Directions
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Revision as of 00:02, 14 September 2010

Research interests

  1. [Microbial Community Analysis]: Bacterial and Fungal Diversity Associated with the Rhizosphere of the nitrogen fixing plant “lupines chamissonis”
  2. [Tommy]
  3. [Jodie]
  4. [Elise]
  5. [Amanda]
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