http://www.openwetware.org/index.php?title=Lum&feed=atom&action=history 2013-05-20T08:31:43Z Revision history for this page on the wiki MediaWiki 1.13.2 http://www.openwetware.org/index.php?title=Lum&diff=470709&oldid=prev 2010-11-05T20:08:06Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:08, 5 November 2010</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 6:</td> <td colspan="2" class="diff-lineno">Line 6:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [[Lum:Elise|Elise]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [[Lum:Elise|Elise]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [[Lum:Amanda|Amanda]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [[Lum:Amanda|Amanda]]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* [[Lum:Michael P.|Michael P.]]</ins></div></td></tr> <!-- diff generator: internal 2013-05-20 08:31:43 --> </table>

Michael R. Pina http://www.openwetware.org/index.php?title=Lum&diff=454331&oldid=prev 2010-09-14T05:07:57Z

<p><span class="autocomment">Research interests</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 05:07, 14 September 2010</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Research interests==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Research interests==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"># </del>[Microbial Community Analysis]: Bacterial and Fungal Diversity Associated with the Rhizosphere of the nitrogen fixing plant <del class="diffchange diffchange-inline">“lupines chamissonis”</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* </ins>[<ins class="diffchange diffchange-inline">[Lum:</ins>Microbial Community Analysis<ins class="diffchange diffchange-inline">| Microbial Community Analysis]</ins>]: Bacterial and Fungal Diversity Associated with the Rhizosphere of the nitrogen fixing plant <ins class="diffchange diffchange-inline">''Lupines chamissonis''</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"># </del>[Tommy]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* </ins>[<ins class="diffchange diffchange-inline">[Lum:Tommy|</ins>Tommy<ins class="diffchange diffchange-inline">]</ins>]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"># </del>[Jodie]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* </ins>[<ins class="diffchange diffchange-inline">[Lum:Jodie|</ins>Jodie<ins class="diffchange diffchange-inline">]</ins>]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"># </del>[Elise]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* </ins>[<ins class="diffchange diffchange-inline">[Lum:Elise|</ins>Elise<ins class="diffchange diffchange-inline">]</ins>]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"># </del>[Amanda]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* </ins>[<ins class="diffchange diffchange-inline">[Lum:</ins>Amanda<ins class="diffchange diffchange-inline">|Amanda]</ins>]</div></td></tr> <!-- diff generator: internal 2013-05-20 08:31:43 --> </table>

Michelle Lum http://www.openwetware.org/index.php?title=Lum&diff=454330&oldid=prev 2010-09-14T05:02:17Z

<p><span class="autocomment">Procedures</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 05:02, 14 September 2010</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==<del class="diffchange diffchange-inline">Procedures==</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==<ins class="diffchange diffchange-inline">Research interests</ins>==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">===Quantification=</del>==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># <del class="diffchange diffchange-inline">'''Instument''' Qubit Fluoromete</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># <ins class="diffchange diffchange-inline">[Microbial Community Analysis]</ins>: <ins class="diffchange diffchange-inline">Bacterial and Fungal Diversity Associated with the Rhizosphere of the nitrogen fixing plant “lupines chamissonis”</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"># '''Setting''' Broad Range</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># <ins class="diffchange diffchange-inline">[Tommy]</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">#* Use Broad Range Standards in BR kit</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># <ins class="diffchange diffchange-inline">[Jodie]</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"># Turn lights off, reagents are light sensitive</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># <ins class="diffchange diffchange-inline">[Elise]</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"># Prepare '''Working Solution'''</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># <ins class="diffchange diffchange-inline">[Amanda]</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">#* 199 µl '''Quant iT Buffer''' </del>: <del class="diffchange diffchange-inline">1 µl '''Quant iT Reagent'''</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#<del class="diffchange diffchange-inline">*199:1 for every sample needed (ex.10 samples will be 2000 µl total)</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"># Prepare Samples</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"># *190 µl working solution + 10 µl standard 1 solution</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"># *190 µl working solution + 10 µl standard 2 solution</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"># *195 µl working solution + 5 µl of DNA</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># <del class="diffchange diffchange-inline">Vortex for 2-3 minutes</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># <del class="diffchange diffchange-inline">Incubate 15 minutes</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># <del class="diffchange diffchange-inline">Follow On Screen Directions</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <!-- diff generator: internal 2013-05-20 08:31:43 --> </table>

Michelle Lum http://www.openwetware.org/index.php?title=Lum&diff=454329&oldid=prev 2010-09-14T04:51:11Z

<p>New page: ==Procedures== ===Quantification=== # '''Instument''' Qubit Fluoromete # '''Setting''' Broad Range #* Use Broad Range Standards in BR kit # Turn lights off, reagents are light sensitive #...</p> <p><b>New page</b></p><div>==Procedures==<br /> ===Quantification===<br /> <br /> # '''Instument''' Qubit Fluoromete<br /> # '''Setting''' Broad Range<br /> #* Use Broad Range Standards in BR kit<br /> # Turn lights off, reagents are light sensitive<br /> # Prepare '''Working Solution'''<br /> #* 199 µl '''Quant iT Buffer''' : 1 µl '''Quant iT Reagent'''<br /> #*199:1 for every sample needed (ex.10 samples will be 2000 µl total)<br /> # Prepare Samples<br /> # *190 µl working solution + 10 µl standard 1 solution<br /> # *190 µl working solution + 10 µl standard 2 solution<br /> # *195 µl working solution + 5 µl of DNA<br /> # Vortex for 2-3 minutes<br /> # Incubate 15 minutes<br /> # Follow On Screen Directions</div>

Michelle Lum