Lum
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Procedures
Quantification
- Instument Qubit Fluoromete
- Setting Broad Range
- Use Broad Range Standards in BR kit
- Turn lights off, reagents are light sensitive
- Prepare Working Solution
- 199 µl Quant iT Buffer : 1 µl Quant iT Reagent
- 199:1 for every sample needed (ex.10 samples will be 2000 µl total)
- Prepare Samples
- *190 µl working solution + 10 µl standard 1 solution
- *190 µl working solution + 10 µl standard 2 solution
- *195 µl working solution + 5 µl of DNA
- Vortex for 2-3 minutes
- Incubate 15 minutes
- Follow On Screen Directions