Lum:Michael P.

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Plant-Microbe Research)
(Protocols)
Line 13: Line 13:
#Remove water with a pipette
#Remove water with a pipette
#Repeat steps 4 & 5 a total of 6 times
#Repeat steps 4 & 5 a total of 6 times
 +
 +
===PCR for nodule bacteria===
 +
*12.5 µL 2x Phusion Mix
 +
*0.25 µL 16S-27F Primer
 +
*0.25 µL 16S-1424R Primer
 +
*4 µL DNA
 +
*3 µL Ultra-pure H20
 +
==SCASM/SCCUR 2010 Poster==
==SCASM/SCCUR 2010 Poster==
[[Media:MP SCASM Poster final mod113.pdf|Poster]]
[[Media:MP SCASM Poster final mod113.pdf|Poster]]

Revision as of 16:48, 26 January 2011

Contents

Plant-Microbe Research

I am currently working on multiple projects in the Lum Lab.

  • One project is to identify the nodulating bacteria associated with Lupinus chamissonis and to determine the role that heavy metals (particularly zinc) play in the symbiosis. This is of importance because of the high levels of heavy metals, such as zinc and copper, found in the Ballona wetlands, (adjacent to Loyola Marymount University) where L. chamissonis grows.
  • Another project is to characterize the nodulation patterns of Caesalpinoid legumes, which are typically understudied.

Protocols

Seed sterilization for L. chamissonis

Make sure all materials are sterile

  1. Lightly scarify seeds on sand paper
  2. Sterilize seeds with 20% bleach for 5 minutes, agitating constantly
  3. Remove bleach using a pipette
  4. Rinse seeds with water for 10 minutes, agitating constantly
  5. Remove water with a pipette
  6. Repeat steps 4 & 5 a total of 6 times

PCR for nodule bacteria

  • 12.5 µL 2x Phusion Mix
  • 0.25 µL 16S-27F Primer
  • 0.25 µL 16S-1424R Primer
  • 4 µL DNA
  • 3 µL Ultra-pure H20

SCASM/SCCUR 2010 Poster

Poster

Personal tools