M465:Biolog assays and Quorum Sensing: Difference between revisions
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Today you will take the final measurements for your Biolog Ecoplates using the spectrophotometer. Afterwards, we will discuss how to analyze these data, and what kinds of questions you might ask with these data using the class dataset. <br> | Today you will take the final measurements for your Biolog Ecoplates using the spectrophotometer. Afterwards, we will discuss how to analyze these data, and what kinds of questions you might ask with these data using the class dataset. <br> | ||
1. | 1. Take your plates out of the incubator. | ||
2. Take a reading at A<sub>590 nm</sub> using the BioTek Synergy H1 <sup>384</sup> plate reader (your instructors will show you how) <BR><BR> | |||
3. Discard your plate in the biohazardous waste containers. <br><br> | |||
'''Carbon source utilization pattern:'''<BR> | |||
How do we analyze our data? You could plot on a bar graph the average A<sub>590 nm</sub> absorbance of the three replicates (with error bars) on the final day of data collection on the y axis and the 31 different carbon sources on the x axis in one figure. (Remember that there is no such thing as a negative value for Absorbance so count anything that is less than zero as zero. Why might you seem to have a negative value?). Should you arrange those carbon sources in the order they are on the BIOLOG plate or is there a better way to organize them on your graph to show your main message(s)? What kind of information do you need in the figure legend? <BR><BR> | |||
Can you think of other ways to illustrate metabolic diversity of your community?<BR><BR> | |||
===Quorum Sensing=== | |||
Revision as of 03:58, 30 August 2016
Finishing Biolog Assays
Today you will take the final measurements for your Biolog Ecoplates using the spectrophotometer. Afterwards, we will discuss how to analyze these data, and what kinds of questions you might ask with these data using the class dataset.
1. Take your plates out of the incubator.
2. Take a reading at A590 nm using the BioTek Synergy H1 384 plate reader (your instructors will show you how)
3. Discard your plate in the biohazardous waste containers.
Carbon source utilization pattern:
How do we analyze our data? You could plot on a bar graph the average A590 nm absorbance of the three replicates (with error bars) on the final day of data collection on the y axis and the 31 different carbon sources on the x axis in one figure. (Remember that there is no such thing as a negative value for Absorbance so count anything that is less than zero as zero. Why might you seem to have a negative value?). Should you arrange those carbon sources in the order they are on the BIOLOG plate or is there a better way to organize them on your graph to show your main message(s)? What kind of information do you need in the figure legend?
Can you think of other ways to illustrate metabolic diversity of your community?
