M465:Fieldtrip and inoculation: Difference between revisions
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Revision as of 11:52, 2 August 2016
Field Trip and Inoculation
In this first lab you will learn:
- How to sample wild *Drosophila melanogaster*
- Aseptic technique: don't contaminate yourself or your cultures!
- The basic equipment and procedures used in microbiological investigation
- Using a lab notebook to record the progress of your experiments
- Inoculating your media for future use
- Drosophila* flies, otherwise known as "fruit flies" or "vinegar flies" or "wine flies", are attracted to decomposing fruit. I turns out that a lot of this has to do with the compounds being produced by the microbes colonizing the fruit as it ferments. Today we will drive to a local winery where we will sweep for fruit flies and also set up baits for them. We will take these samples back to the lab and use them to inoculate two different kinds of medium, Luria Bertani (LB) agar and de Man, Rogosa, Sharpe (MRS) agar. Each of these medias are selective for specific microbes, all heterotrophic. The LB agar will select enteric microbes, such as *Enterobacter*, commonly associated with the fruit fly. The MRS agar will select for lactic acid microbes, such as *Lactobacillus* and *Enterococcus* species also associated with the fruit fly.
Trip to the winery
Please arrive on time in JH 022. We will drive to the winery together. If you are able to drive, let your instructor know ahead of time.
