M9 medium: Difference between revisions
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==Introduction== | |||
M9 media is a minimal media used for bacterial cultures. It has the advantage of being cheap and has a very low autofluorescence. M9 media can be supplemented to produce higher growth rates. | |||
==Specific Protocols== | |||
*[[M9 media/minimal|M9 minimal media]] | |||
*[[M9 media/supplemented|M9 supplmented media]] | |||
• [[M9 supplemented media|<font style="color:#000000">M9 supplemented media</font>]] <BR> | |||
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Revision as of 07:18, 30 March 2006
Introduction
M9 media is a minimal media used for bacterial cultures. It has the advantage of being cheap and has a very low autofluorescence. M9 media can be supplemented to produce higher growth rates.
Specific Protocols
M9 medium also exists in various flavors:
M9-glu contains glucose: In the basic recipe take 800 ml sterile water and supplement with 100 ml 20% (w:v) sterile filtered glucose before adding M9 salts, magnesium and calcium salts.
M9-CA contains casamino acids: In the basic recipe take 800 ml sterile water and supplement with 100 ml 10% (w:v) autoclaved casamino acids (in water) before adding M9 salts, magnesium and calcium salts.
M9-CAglu contains both: In the basic recipe take 700 ml sterile water and supplement with 100 ml 20% glucose and 100 ml casamino acids before adding M9 salts, magnesium and calcium salts.
Warning: salts that are added last often tend to precipitate. I didn't bother about it too much as obviously it didn't influence bacterial growth. There might be a way to avoid precipitation (eventually adding magnesium and calcium first) but I didn't investigate this in detail.