|
|
| Line 7: |
Line 7: |
| *[[M9 media/supplemented|M9 supplmented media]] | | *[[M9 media/supplemented|M9 supplmented media]] |
| • [[M9 supplemented media|<font style="color:#000000">M9 supplemented media</font>]] <BR> | | • [[M9 supplemented media|<font style="color:#000000">M9 supplemented media</font>]] <BR> |
|
| |
|
| |
| M9 medium also exists in various flavors:
| |
|
| |
| '''M9-glu''' contains glucose: In the basic recipe take 800 ml sterile water and supplement with 100 ml 20% (w:v) sterile filtered glucose before adding M9 salts, magnesium and calcium salts.
| |
|
| |
| '''M9-CA''' contains casamino acids: In the basic recipe take 800 ml sterile water and supplement with 100 ml 10% (w:v) autoclaved casamino acids (in water) before adding M9 salts, magnesium and calcium salts.
| |
|
| |
| '''M9-CAglu''' contains both: In the basic recipe take 700 ml sterile water and supplement with 100 ml 20% glucose and 100 ml casamino acids before adding M9 salts, magnesium and calcium salts.
| |
|
| |
| ''Warning: salts that are added last often tend to precipitate. I didn't bother about it too much as obviously it didn't influence bacterial growth. There might be a way to avoid precipitation (eventually adding magnesium and calcium first) but I didn't investigate this in detail.''
| |
Revision as of 07:19, 30 March 2006
Introduction
M9 media is a minimal media used for bacterial cultures. It has the advantage of being cheap and has a very low autofluorescence. M9 media can be supplemented to produce higher growth rates.
Specific Protocols
• M9 supplemented media
