M9 medium/minimal

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Current revision (13:20, 20 April 2007) (view source)
 
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We used to prepare the '''plain M9 medium''' as follows:
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==Recipe==
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*1x [[M9 salts]]
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*2mM MgSO<sub>4</sub>
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*0.1mM CaCl<sub>2</sub>
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*0.4% carbon source (e.g. glycerol, glucose, etc.)
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*In sterile H<sub>2</sub>O
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{|
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==Specific Protocols==
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| 900 ml sterile water
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*[[FCCT Biochemistry Lab:M9 medium/minimal]]
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|-
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*[[Endy:M9 medium/minimal]] <-same recipe as above but includes lab specific details about our stocks.
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| 100 ml M9 salts stock solution
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| 1 ml autoclaved 1 M MgSO<sub>4</sub>
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| 0.1 ml autoclaved 1 M CaCl<sub>2</sub>
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|}
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==References==
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<biblio>
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Sambrook isbn=0879695773
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</biblio>
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[[Category:Material]] [[Category:Escherichia coli]]
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'''M9 stock solution''' (10x) is:
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{|
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| 60 g Na<sub>2</sub>HPO<sub>4</sub> x 7 H<sub>2</sub>O
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| 30 g KH<sub>2</sub>PO<sub>4</sub>
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| 5 g NaCl
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| 10 g NH<sub>4</sub>Cl
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| add water to 1000 ml and autoclave
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|}
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==Notes==
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''Note 1: salts that are added last often tend to precipitate. I didn't bother about it too much as obviously it didn't influence bacterial growth. There might be a way to avoid precipitation (eventually adding magnesium and calcium first) but I didn't investigate this in detail.''
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''Note 2: The M9 stock solution here is essentially as in the [http://openwetware.org/wiki/M9_salts M9 salts] recipe just that the concentration is about half of that. Nevertheless, bacteria are normally OK with this.''
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Current revision

Recipe

  • 1x M9 salts
  • 2mM MgSO4
  • 0.1mM CaCl2
  • 0.4% carbon source (e.g. glycerol, glucose, etc.)
  • In sterile H2O

Specific Protocols

References

  1. Joseph Sambrook, David W. Russell. Molecular cloning. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory, 2001. isbn:0879695773. [Sambrook]
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