M9 medium/minimal

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We used to prepare the '''plain M9 medium''' as follows:
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==Recipe==
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{|
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==Specific Protocols==
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| 900 ml sterile water
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*[[FCCT Biochemistry Lab:M9 medium/minimal]]
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| 100 ml M9 salts stock solution
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| 1 ml autoclaved 1 M MgSO<sub>4</sub>
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| 0.1 ml autoclaved 1 M CaCl<sub>2</sub>
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|}
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==References==
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'''M9 stock solution''' (10x) is:
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| 60 g Na<sub>2</sub>HPO<sub>4</sub> x 7 H<sub>2</sub>O
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| 30 g KH<sub>2</sub>PO<sub>4</sub>
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| 5 g NaCl
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| 10 g NH<sub>4</sub>Cl
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| add water to 1000 ml and autoclave
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|}
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==Notes==
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''Note 1: salts that are added last often tend to precipitate. I didn't bother about it too much as obviously it didn't influence bacterial growth. There might be a way to avoid precipitation (eventually adding magnesium and calcium first) but I didn't investigate this in detail.''
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''Note 2: The M9 stock solution here is essentially as in the [http://openwetware.org/wiki/M9_salts M9 salts] recipe just that the concentration is about half of that. Nevertheless, bacteria are normally OK with this.''
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Revision as of 21:29, 13 September 2006

Recipe

Specific Protocols

References

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