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| We used to prepare the '''plain M9 medium''' as follows:
| | ==Recipe== |
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| |
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| {|
| | ==Specific Protocols== |
| | 900 ml sterile water
| | *[[FCCT Biochemistry Lab:M9 medium/minimal]] |
| |-
| |
| | 100 ml M9 salts stock solution
| |
| |-
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| | 1 ml autoclaved 1 M MgSO<sub>4</sub>
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| |-
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| | 0.1 ml autoclaved 1 M CaCl<sub>2</sub>
| |
| |}
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| | | ==References== |
| | |
| '''M9 stock solution''' (10x) is:
| |
| {|
| |
| | 60 g Na<sub>2</sub>HPO<sub>4</sub> x 7 H<sub>2</sub>O
| |
| |-
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| | 30 g KH<sub>2</sub>PO<sub>4</sub>
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| |-
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| | 5 g NaCl
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| |-
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| | 10 g NH<sub>4</sub>Cl
| |
| |-
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| | add water to 1000 ml and autoclave
| |
| |}
| |
| | |
| ==Notes== | |
| ''Note 1: salts that are added last often tend to precipitate. I didn't bother about it too much as obviously it didn't influence bacterial growth. There might be a way to avoid precipitation (eventually adding magnesium and calcium first) but I didn't investigate this in detail.''
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| ''Note 2: The M9 stock solution here is essentially as in the [http://openwetware.org/wiki/M9_salts M9 salts] recipe just that the concentration is about half of that. Nevertheless, bacteria are normally OK with this.''
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