M9 medium/minimal: Difference between revisions

Latest revision as of 10:20, 20 April 2007

FCCT Biochemistry Lab:M9 medium/minimal
Endy:M9 medium/minimal <-same recipe as above but includes lab specific details about our stocks.

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-We used to prepare the '''plain M9 medium''' as follows:
+==Recipe==
+*1x [[M9 salts]]
+*2mM MgSO<sub>4</sub>
+*0.1mM CaCl<sub>2</sub>
+*0.4% carbon source (e.g. glycerol, glucose, etc.)
+*In sterile H<sub>2</sub>O
-{|
+==Specific Protocols==
-| 900 ml sterile water
+*[[FCCT Biochemistry Lab:M9 medium/minimal]]
-|-
+*[[Endy:M9 medium/minimal]] <-same recipe as above but includes lab specific details about our stocks.
-| 100 ml M9 salts stock solution
-|-
-| 1 ml autoclaved 1 M MgSO<sub>4</sub>
-|-
-| 0.1 ml autoclaved 1 M CaCl<sub>2</sub>
-|}
+==References==
+<biblio>
+Sambrook isbn=0879695773
+</biblio>
+[[Category:Material]] [[Category:Escherichia coli]]
-'''M9 stock solution''' (10x) is:
-{|
-| 60 g Na<sub>2</sub>HPO<sub>4</sub> x 7 H<sub>2</sub>O
-|-
-| 30 g KH<sub>2</sub>PO<sub>4</sub>
-|-
-| 5 g NaCl
-|-
-| 10 g NH<sub>4</sub>Cl
-|-
-| add water to 1000 ml and autoclave
-|}
-==Notes==
-''Note 1: salts that are added last often tend to precipitate. I didn't bother about it too much as obviously it didn't influence bacterial growth. There might be a way to avoid precipitation (eventually adding magnesium and calcium first) but I didn't investigate this in detail.''
-''Note 2: The M9 stock solution here is essentially as in the [http://openwetware.org/wiki/M9_salts M9 salts] recipe just that the concentration is about half of that. Nevertheless, bacteria are normally OK with this.''