M9 medium/minimal

From OpenWetWare

< M9 medium
Revision as of 14:17, 30 March 2006 by MDolinar (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

We used to prepare the plain M9 medium as follows:

900 ml sterile water
100 ml M9 salts stock solution
1 ml autoclaved 1 M MgSO4
0.1 ml autoclaved 1 M CaCl2

M9 stock solution (10x) is:

60 g Na2HPO4 x 7 H2O
30 g KH2PO4
5 g NaCl
10 g NH4Cl
add water to 1000 ml and autoclave


Note 1: salts that are added last often tend to precipitate. I didn't bother about it too much as obviously it didn't influence bacterial growth. There might be a way to avoid precipitation (eventually adding magnesium and calcium first) but I didn't investigate this in detail.

Note 2: The M9 stock solution here is essentially as in the M9 salts recipe just that the concentration is about half of that. Nevertheless, bacteria are normally OK with this.

Personal tools