M9 medium/supplemented

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(Knight & Endy Lab)
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**[[Knight:M9 supplemented media|Knight lab protocol]]
**[[Knight:M9 supplemented media|Knight lab protocol]]
**[[Endy:M9 media/supplemented|Endy lab protocol]]
**[[Endy:M9 media/supplemented|Endy lab protocol]]
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*[[Julius B. Lucks/M9 Supplemented Minimal Media]]
[[Category:Material]] [[Category:Escherichia coli]]
[[Category:Material]] [[Category:Escherichia coli]]

Revision as of 19:38, 3 April 2008

M9 medium also exists in various flavors:

M9-glu contains glucose: In the basic recipe take 800 ml sterile water and supplement with 100 ml 20% (w:v) sterile filtered glucose before adding M9 salts, magnesium and calcium salts. [This and following instructions refer to the basic M9 medium recipe.]

M9-CA contains casamino acids: In the basic recipe take 800 ml sterile water and supplement with 100 ml 10% (w:v) autoclaved casamino acids (in water) before adding M9 salts, magnesium and calcium salts.

M9-CAglu contains both: In the basic recipe take 700 ml sterile water and supplement with 100 ml 20% glucose and 100 ml casamino acids before adding M9 salts, magnesium and calcium salts.


Specific Recipes

Knight & Endy Lab

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