M9 medium/supplemented

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Current revision (22:06, 8 April 2008) (view source)
 
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M9 medium also exists in various flavors:
M9 medium also exists in various flavors:
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'''M9-glu''' contains glucose: In the basic recipe take 800 ml sterile water and supplement with 100 ml 20% (w:v) sterile filtered glucose before adding M9 salts, magnesium and calcium salts.
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'''M9-glu''' contains glucose: In the basic recipe take 800 ml sterile water and supplement with 100 ml 20% (w:v) sterile filtered glucose before adding M9 salts, magnesium and calcium salts. [This and following instructions refer to the [http://openwetware.org/wiki/M9_medium/minimal basic M9 medium] recipe.]
'''M9-CA''' contains casamino acids: In the basic recipe take 800 ml sterile water and supplement with 100 ml 10% (w:v) autoclaved casamino acids (in water) before adding M9 salts, magnesium and calcium salts.
'''M9-CA''' contains casamino acids: In the basic recipe take 800 ml sterile water and supplement with 100 ml 10% (w:v) autoclaved casamino acids (in water) before adding M9 salts, magnesium and calcium salts.
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'''M9-CAglu''' contains both: In the basic recipe take 700 ml sterile water and supplement with 100 ml 20% glucose and 100 ml casamino acids before adding M9 salts, magnesium and calcium salts.
'''M9-CAglu''' contains both: In the basic recipe take 700 ml sterile water and supplement with 100 ml 20% glucose and 100 ml casamino acids before adding M9 salts, magnesium and calcium salts.
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''Warning: salts that are added last often tend to precipitate. I didn't bother about it too much as obviously it didn't influence bacterial growth. There might be a way to avoid precipitation (eventually adding magnesium and calcium first) but I didn't investigate this in detail.''
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==Specific Recipes==
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===Knight & Endy Lab===
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*[[M9 medium/minimal|M9 minimal medium]] supplemented with 0.2% Casamino acids and 1mM Thiamine Hydrochloride.
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**[[Knight:M9 supplemented media|Knight lab protocol]]
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**[[Endy:M9 media/supplemented|Endy lab protocol]]
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***[[Julius B. Lucks/M9 Supplemented Minimal Media|Lucks modified Endy recipe]]
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***[[User:Dileep_D._Monie/Recipes/Supplemented_M9|Monie modified Endy recipe]]
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[[Category:Material]] [[Category:Escherichia coli]]

Current revision

M9 medium also exists in various flavors:

M9-glu contains glucose: In the basic recipe take 800 ml sterile water and supplement with 100 ml 20% (w:v) sterile filtered glucose before adding M9 salts, magnesium and calcium salts. [This and following instructions refer to the basic M9 medium recipe.]

M9-CA contains casamino acids: In the basic recipe take 800 ml sterile water and supplement with 100 ml 10% (w:v) autoclaved casamino acids (in water) before adding M9 salts, magnesium and calcium salts.

M9-CAglu contains both: In the basic recipe take 700 ml sterile water and supplement with 100 ml 20% glucose and 100 ml casamino acids before adding M9 salts, magnesium and calcium salts.


Specific Recipes

Knight & Endy Lab

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