M9 medium/supplemented

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'''M9-CAglu''' contains both: In the basic recipe take 700 ml sterile water and supplement with 100 ml 20% glucose and 100 ml casamino acids before adding M9 salts, magnesium and calcium salts.
'''M9-CAglu''' contains both: In the basic recipe take 700 ml sterile water and supplement with 100 ml 20% glucose and 100 ml casamino acids before adding M9 salts, magnesium and calcium salts.
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==Specific Protocols==
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*[[Knight:M9 supplemented media|Knight and Endy lab protocol]]
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==Notes==
''Warning: salts that are added last often tend to precipitate. I didn't bother about it too much as obviously it didn't influence bacterial growth. There might be a way to avoid precipitation (eventually adding magnesium and calcium first) but I didn't investigate this in detail.''
''Warning: salts that are added last often tend to precipitate. I didn't bother about it too much as obviously it didn't influence bacterial growth. There might be a way to avoid precipitation (eventually adding magnesium and calcium first) but I didn't investigate this in detail.''

Revision as of 10:21, 30 March 2006

M9 medium also exists in various flavors:

M9-glu contains glucose: In the basic recipe take 800 ml sterile water and supplement with 100 ml 20% (w:v) sterile filtered glucose before adding M9 salts, magnesium and calcium salts.

M9-CA contains casamino acids: In the basic recipe take 800 ml sterile water and supplement with 100 ml 10% (w:v) autoclaved casamino acids (in water) before adding M9 salts, magnesium and calcium salts.

M9-CAglu contains both: In the basic recipe take 700 ml sterile water and supplement with 100 ml 20% glucose and 100 ml casamino acids before adding M9 salts, magnesium and calcium salts.


Specific Protocols


Notes

Warning: salts that are added last often tend to precipitate. I didn't bother about it too much as obviously it didn't influence bacterial growth. There might be a way to avoid precipitation (eventually adding magnesium and calcium first) but I didn't investigate this in detail.

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