M9 medium/supplemented

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(deleted note, added link to basic M9 medium recipe)
Line 1: Line 1:
M9 medium also exists in various flavors:
M9 medium also exists in various flavors:
-
'''M9-glu''' contains glucose: In the basic recipe take 800 ml sterile water and supplement with 100 ml 20% (w:v) sterile filtered glucose before adding M9 salts, magnesium and calcium salts.
+
'''M9-glu''' contains glucose: In the basic recipe take 800 ml sterile water and supplement with 100 ml 20% (w:v) sterile filtered glucose before adding M9 salts, magnesium and calcium salts. [This and following instructions refer to the [http://openwetware.org/wiki/M9_medium/minimal basic M9 medium] recipe.]
'''M9-CA''' contains casamino acids: In the basic recipe take 800 ml sterile water and supplement with 100 ml 10% (w:v) autoclaved casamino acids (in water) before adding M9 salts, magnesium and calcium salts.
'''M9-CA''' contains casamino acids: In the basic recipe take 800 ml sterile water and supplement with 100 ml 10% (w:v) autoclaved casamino acids (in water) before adding M9 salts, magnesium and calcium salts.
Line 10: Line 10:
==Specific Protocols==
==Specific Protocols==
*[[Knight:M9 supplemented media|Knight and Endy lab protocol]]
*[[Knight:M9 supplemented media|Knight and Endy lab protocol]]
-
 
-
 
-
==Notes==
 
-
''Warning: salts that are added last often tend to precipitate. I didn't bother about it too much as obviously it didn't influence bacterial growth. There might be a way to avoid precipitation (eventually adding magnesium and calcium first) but I didn't investigate this in detail.''
 

Revision as of 14:21, 30 March 2006

M9 medium also exists in various flavors:

M9-glu contains glucose: In the basic recipe take 800 ml sterile water and supplement with 100 ml 20% (w:v) sterile filtered glucose before adding M9 salts, magnesium and calcium salts. [This and following instructions refer to the basic M9 medium recipe.]

M9-CA contains casamino acids: In the basic recipe take 800 ml sterile water and supplement with 100 ml 10% (w:v) autoclaved casamino acids (in water) before adding M9 salts, magnesium and calcium salts.

M9-CAglu contains both: In the basic recipe take 700 ml sterile water and supplement with 100 ml 20% glucose and 100 ml casamino acids before adding M9 salts, magnesium and calcium salts.


Specific Protocols

Personal tools