M9 medium/supplemented
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| - | ==Specific | + | ==Specific Recipes== |
| + | ===Knight & Endy Lab=== | ||
| + | *[[M9 medium/minimal|M9 minimal medium]] supplemented with 0.2% Casamino acids and 1mM Thiamine Hydrochloride. | ||
*[[Knight:M9 supplemented media|Knight and Endy lab protocol]] | *[[Knight:M9 supplemented media|Knight and Endy lab protocol]] | ||
*[[Endy:M9 media/supplemented|Endy: M9 supplemented media]] | *[[Endy:M9 media/supplemented|Endy: M9 supplemented media]] | ||
Revision as of 21:40, 13 September 2006
M9 medium also exists in various flavors:
M9-glu contains glucose: In the basic recipe take 800 ml sterile water and supplement with 100 ml 20% (w:v) sterile filtered glucose before adding M9 salts, magnesium and calcium salts. [This and following instructions refer to the basic M9 medium recipe.]
M9-CA contains casamino acids: In the basic recipe take 800 ml sterile water and supplement with 100 ml 10% (w:v) autoclaved casamino acids (in water) before adding M9 salts, magnesium and calcium salts.
M9-CAglu contains both: In the basic recipe take 700 ml sterile water and supplement with 100 ml 20% glucose and 100 ml casamino acids before adding M9 salts, magnesium and calcium salts.
Specific Recipes
Knight & Endy Lab
- M9 minimal medium supplemented with 0.2% Casamino acids and 1mM Thiamine Hydrochloride.
- Knight and Endy lab protocol
- Endy: M9 supplemented media
