M9 salts

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m (h -> H in NH4Cl for accuracy)
Line 3: Line 3:
*15 g KH<sub>2</sub>PO<sub>4</sub>
*15 g KH<sub>2</sub>PO<sub>4</sub>
*2.5 g NaCl
*2.5 g NaCl
-
*5 g Nh<sub>4</sub>Cl
+
*5 g NH<sub>4</sub>Cl
dissolved in deionized water to a final volume of 1 L
dissolved in deionized water to a final volume of 1 L

Revision as of 15:43, 26 January 2006

5X M9 salts contains:

  • 64 g Na2HPO4.7H2O
  • 15 g KH2PO4
  • 2.5 g NaCl
  • 5 g NH4Cl

dissolved in deionized water to a final volume of 1 L

Source

Adapted From:

J. Sambrook, D.W. Russell, Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, New York, ed. 3, 2001) pg. A2.2

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