MEG
From OpenWetWare
(Difference between revisions)
(→Molecules of Equivalent GFP (MEG)) |
Current revision (17:06, 30 June 2006) (view source) |
||
| (4 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
==Molecules of Equivalent GFP (MEG)== | ==Molecules of Equivalent GFP (MEG)== | ||
| - | + | [[Image:Cconboy-Flow Calibration.png|200px]] | |
| - | + | [Image:Cconboy-Flow Calibration.png|200px] | |
| - | + | Fig.1. Spherotech yellow fluorescent particles (FP-0852-2) were calibrated to GFP copies per cell as measured by quanitiative western blot. The data series "Cells" includes samples from four independent chemostat cultures. Each contained MC4100 with a plasmid-based GFP generator: pSB4A3-I7109, pSB4A3-I7107, pSB3K3-I7109 or pSB3K3-I7107, as noted. | |
| - | The data series "Cells" includes samples from four independent chemostat cultures. Each contained MC4100 with a plasmid-based GFP generator: pSB4A3-I7109, pSB4A3-I7107, pSB3K3-I7109 or pSB3K3-I7107, as noted. | + | |
===Standard curve=== | ===Standard curve=== | ||
| Line 19: | Line 18: | ||
===Reference=== | ===Reference=== | ||
| - | This work was done by [[Caitlin Conboy]] and [[ | + | This work was done by [[Caitlin Conboy]] and [[Jennifer Braff]]. Thorough documentation to come... |
Current revision
Contents |
Molecules of Equivalent GFP (MEG)
[Image:Cconboy-Flow Calibration.png|200px]
Fig.1. Spherotech yellow fluorescent particles (FP-0852-2) were calibrated to GFP copies per cell as measured by quanitiative western blot. The data series "Cells" includes samples from four independent chemostat cultures. Each contained MC4100 with a plasmid-based GFP generator: pSB4A3-I7109, pSB4A3-I7107, pSB3K3-I7109 or pSB3K3-I7107, as noted.
Standard curve
[Fluorescence intensity] = 0.0025*[GFP copies/cell]-14.416
R^2 = 0.9684
FP-0852-2 MEG
From the standard curve above, FP-0852-2 beads represent ~370,000 MEG (+/-40%)
Reference
This work was done by Caitlin Conboy and Jennifer Braff. Thorough documentation to come...
