MES
From OpenWetWare
2--(N-morpholino)ethanesulfonic acid, CAS 4432-31-9 [1][2][3]. Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range.
- pKa at 25C of 6.10 (5.97 at 37C)
- usable buffering range of 5.5 to 6.7
- molecular weight 195.24
- ΔpKa/ΔT = -.011
Buffers are typically 1 M, prepared by neutralizing MES with sodium hydroxide.
- Good NE, Winget GD, Winter W, Connolly TN, Izawa S, and Singh RM. Hydrogen ion buffers for biological research. Biochemistry. 1966 Feb;5(2):467-77. DOI:10.1021/bi00866a011 |
- Good NE and Izawa S. Hydrogen ion buffers. Methods Enzymol. 1972;24:53-68. DOI:10.1016/0076-6879(72)24054-x |
- Blanchard JS. Buffers for enzymes. Methods Enzymol. 1984;104:404-14. DOI:10.1016/s0076-6879(84)04107-0 |
