MIT Synbio:IAP
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2 Intro classes: 1 day with two info sessions for people to come and see if they want to take the class or not
- Take place before EHS training
Class 0: for people who haven't taken 7.012: DNA What is synthetic biology
- things that have been done in the past
- potential projects
- Circuits
- what you can do.
What are biobricks
- registry
circuit concepts, circuit design (and/ or gates, NOR gates, etc.)/ feedback iGEM What they're doing: bioluminescence Techniques to teach:
- Lab tour: pipetting, sterile
- (Minipreps)
- We need to make plates, gels
- Labeling
1. Digestion: 1.5 hr 2. Ligation: 1.5 hr, do at the end 3. Gel extract + nanodrop: 20 min prep, 30 min run; 10 min cut, 20 min spin, 5 min nanodrop: 1.5 hr 4. Transformation: 1 hr, 1 hr grow, 20 min plating 5. Picking colonies 20 min--> cell stock--> grow up (us) 6. Miniprep: 1.5 hr, nanodrop 5 min 7. Restriction map