MIT Synbio:IAP

2 Intro classes: 1 day with two info sessions for people to come and see if they want to take the class or not

• Take place before EHS training

Class 0: for people who haven't taken 7.012: DNA What is synthetic biology

• things that have been done in the past
• potential projects
• Circuits
• what you can do.

What are biobricks

• registry

circuit concepts, circuit design (and/ or gates, NOR gates, etc.)/ feedback iGEM
What they're doing: bioluminescence

Techniques to teach:

• Lab tour: pipetting, sterile
• (Minipreps)
• We need to make plates, gels
• Labeling

1. Digestion: 1.5 hr 2. Ligation: 1.5 hr, do at the end 3. Gel extract + nanodrop: 20 min prep, 30 min run; 10 min cut, 20 min spin, 5 min nanodrop: 1.5 hr 4. Transformation: 1 hr, 1 hr grow, 20 min plating 5. Picking colonies 20 min--> cell stock--> grow up (us) 6. Miniprep: 1.5 hr, nanodrop 5 min 7. Restriction map