MIT Synbio:IAP2011-Curriculum: Difference between revisions

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<p><table width=70% align=center><tr><td><ul id="menu"><br><li class="current">1.7.11 | Class 1: Intro to Synthetic Biology</ul>
<p><table width=70% align=center><tr><td><ul id="menu"><br><li class="current">1.7.11 | Class 1: Intro to Synthetic Biology (1/2)</ul>
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     * Quick review of genetic engineering
     * Quick review of genetic engineering
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<p><table width=70% align=center><tr><td><ul id="menu"><br><li class="current">1.10.11 | Class 2: Intro to Synthetic Biology (2/2)</ul>
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    * Why E. coli?
          o E. coli biology and characterization
          o Safety and relative ease of use
    * Background on luciferase
          o Natural form
          o Extraction and isolation of gene. Registry entry
          o Short on biological mechanism
    * Background on Inducible Promoter (assuming AHL-luxR driven)
          o History of quorum sensing
          o Short on biological mechanism
    * Short on cloning technique sequence
          o Very, very broad. As in transform=put plasmid in cell.
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<p><table width=70% align=center><tr><td><ul id="menu"><br><li class="current">1.10.11 | Class 2: Lab Basics and EHS Training (MANDATORY)</ul>
<p><table width=70% align=center><tr><td><ul id="menu"><br><li class="current">1.11.11 | Class 3: Lab Basics and EHS Training (MANDATORY)</ul>
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Class 3: Lab basics, EHS training
• EHS Training, Mandatory
• EHS Training, Mandatory
• Need to complete on their own: gen chem, haz waste, read chem hygiene plan
• Need to complete on their own: gen chem, haz waste, read chem hygiene plan
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<p><table width=70% align=center><tr><td><ul id="menu"><br><li class="current">1.12.11 | Class 4: Restriction digest (MANDATORY)</ul>
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    * Journal club/ speaker during down time with RE digest
    * (We phosphatase overnight)
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<p><table width=70% align=center><tr><td><ul id="menu"><br><li class="current">1.13.11 | Class 5: Gel Extraction, Ligation (overnight)</ul>
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    * (We will have back up RE digest products for gel extraction)
    * Journal club/ speaker after ligation
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<p><table width=70% align=center><tr><td><ul id="menu"><br><li class="current">1.14.11 | Class 6: Gel Extraction, Ligation (overnight)</ul>
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    * (We will have back up RE digest products for gel extraction)
    * Journal club/ speaker after ligation
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Revision as of 12:04, 6 December 2010

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   * Life’s central dogma. What are the roles of DNA, mRNA, proteins?
         o DNA’s structure. DNA replication
          Transcription and translation mechanics
         o Proteins as phenotype
   * Introduction to genetic engineering principles
         o Plasmids & vectors vs. chromosomal DNA
         o Concepts behind restriction enzymes, gene transfer
         o Some historical examples: insulin, etc. 


   * Quick review of genetic engineering
   * Synthetic Biology
         o What’s new?
               + Bottom-up construction
               + Standardization: analogues to electrical circuits
         o BioBricks & the Registry
               + What’s in a BioBrick?
               + How can they be put together?
               + Where are they kept?
         o Emergent Circuit Complexity
               + Rational design
               + Circuit functions (and/or, NOR gates, feedback)
               + iGEM 
   * Teaser: You’re making a bacterial lamp!


   * Why E. coli?
         o E. coli biology and characterization
         o Safety and relative ease of use
   * Background on luciferase
         o Natural form
         o Extraction and isolation of gene. Registry entry
         o Short on biological mechanism

   * Background on Inducible Promoter (assuming AHL-luxR driven)
         o History of quorum sensing
         o Short on biological mechanism

   * Short on cloning technique sequence
         o Very, very broad. As in transform=put plasmid in cell.

• EHS Training, Mandatory • Need to complete on their own: gen chem, haz waste, read chem hygiene plan 

   * Before anything is done, lab basics :]
         o Tour!
         o Hygiene and sterile techniques, safety
               + Burnboxes, safety shower, tips containers, ethanol/bleach/alconox
               + Review EHS training. Liquid/chemical/biosafety disposal
         o Pipet basics (how to hold one, pushing in a tip, liquid, tip removal)
               + Also brief foray into mechanical pipets
         o Flame. Why do we use it? How to use it?


   * Journal club/ speaker during down time with RE digest
   * (We phosphatase overnight)


   * (We will have back up RE digest products for gel extraction)
   * Journal club/ speaker after ligation


   * (We will have back up RE digest products for gel extraction)
   * Journal club/ speaker after ligation

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