<html>
<head>
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<head>
<link rel="stylesheet" href="http://www.cssmenumaker.com/menus/022/menu_style.css" type="text/css" />
<style type="text/css">
div.announce
{
width:100%;
border:0;
margin:0px;
}
</style>
</head>
</html>
More complete curriculum draft
* Life’s central dogma. What are the roles of DNA, mRNA, proteins?
o DNA’s structure. DNA replication
Transcription and translation mechanics
o Proteins as phenotype
* Introduction to genetic engineering principles
o Plasmids & vectors vs. chromosomal DNA
o Concepts behind restriction enzymes, gene transfer
o Some historical examples: insulin, etc.
|
* Quick review of genetic engineering
* Synthetic Biology
o What’s new?
+ Bottom-up construction
+ Standardization: analogues to electrical circuits
o BioBricks & the Registry
+ What’s in a BioBrick?
+ How can they be put together?
+ Where are they kept?
o Emergent Circuit Complexity
+ Rational design
+ Circuit functions (and/or, NOR gates, feedback)
+ iGEM
* Teaser: You’re making a bacterial lamp!
|
* Why E. coli?
o E. coli biology and characterization
o Safety and relative ease of use
* Background on luciferase
o Natural form
o Extraction and isolation of gene. Registry entry
o Short on biological mechanism
* Background on Inducible Promoter (assuming AHL-luxR driven)
o History of quorum sensing
o Short on biological mechanism
* Short on cloning technique sequence
o Very, very broad. As in transform=put plasmid in cell.
|
• EHS Training, Mandatory
• Need to complete on their own: gen chem, haz waste, read chem hygiene plan
* Before anything is done, lab basics :]
o Tour!
o Hygiene and sterile techniques, safety
+ Burnboxes, safety shower, tips containers, ethanol/bleach/alconox
+ Review EHS training. Liquid/chemical/biosafety disposal
o Pipet basics (how to hold one, pushing in a tip, liquid, tip removal)
+ Also brief foray into mechanical pipets
o Flame. Why do we use it? How to use it?
|
* Journal club/ speaker during down time with RE digest
* (We phosphatase overnight)
|
* (We will have back up RE digest products for gel extraction)
* Journal club/ speaker after ligation
|
* (We will have back up RE digest products for gel extraction)
* Journal club/ speaker after ligation
|
