MOPS
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(Some OWW protocols which use MOPS) |
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==Recipe for 10x MOPS buffer== | ==Recipe for 10x MOPS buffer== | ||
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* [[Knight:NuPAGE electrophoresis]] | * [[Knight:NuPAGE electrophoresis]] | ||
* [[Sauer:bis-Tris SDS-PAGE, the very best]] | * [[Sauer:bis-Tris SDS-PAGE, the very best]] | ||
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| + | == External links == | ||
| + | * [http://en.wikipedia.org/wiki/MOPS Wikipedia MOPS] | ||
Revision as of 11:38, 2 March 2009
Recipe for 10x MOPS buffer
- 41.2g 3-(N-morpholino) propanesulfonic acid (MOPS)
- 10.9g Sodium Acetate, 3- hydrate
- 3.7g EDTA, sodium salt
- add 800ml of nuclease free distilled water; mix to dissolve
- adjust to pH 7 with NaOH (prepared in nuclease free distilled water)
- fill to the final volume of 1000 ml
- filter sterilise
- store at room temperature
- protect from light; do not use if the solution appears yellow
Some OWW protocols which use MOPS
- Jacobs:Protocol RNA Agarose Gel
- Knight:NuPAGE electrophoresis
- Sauer:bis-Tris SDS-PAGE, the very best
