MOPS: Difference between revisions
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==Recipe for 10x MOPS buffer== | ==Recipe for 10x MOPS buffer== | ||
[[Image:800px-MOPS.png|right|200px|chemical structure of MOPS, 3-(N-morpholino) propanesulfonic acid]] | |||
* 41.2g 3-(N-morpholino) propanesulfonic acid (MOPS) | * 41.2g 3-(N-morpholino) propanesulfonic acid (MOPS) | ||
Revision as of 08:44, 2 March 2009
MOPS is the common name for the buffering compound in MOPS buffer. MOPS stands for 3-(N-morpholino) propanesulfonic acid and with a pKa of 7.20, MOPS is an good buffer for many biological systems at almost neutral pH. HEPES is a chemically similar pH buffering compound.
Recipe for 10x MOPS buffer
- 41.2g 3-(N-morpholino) propanesulfonic acid (MOPS)
- 10.9g Sodium Acetate, 3- hydrate
- 3.7g EDTA, sodium salt
- add 800ml of nuclease free distilled water; mix to dissolve
- adjust to pH 7 with NaOH (prepared in nuclease free distilled water)
- fill to the final volume of 1000 ml
- filter sterilise
- store at room temperature
- protect from light; do not use if the solution appears yellow
Some OWW protocols which use MOPS
- Jacobs:Protocol RNA Agarose Gel
- Knight:NuPAGE electrophoresis
- Sauer:bis-Tris SDS-PAGE, the very best
