MOPS - Revision history

Christopher C Vanlang at 09:58, 10 January 2012

2012-01-10T09:58:34Z

←Older revision		Revision as of 09:58, 10 January 2012
Line 44:		Line 44:
	* [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=M5755\|SIGMA&N5=SEARCH_CONCAT_PNO\|BRAND_KEY&F=SPEC premade MOPS 10x from Sigma]		* [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=M5755\|SIGMA&N5=SEARCH_CONCAT_PNO\|BRAND_KEY&F=SPEC premade MOPS 10x from Sigma]
	* [http://cshprotocols.cshlp.org/cgi/content/full/2006/3/pdb.rec8332?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=mops&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT&text_only=true CSH Protocols MOPS] [[Image:Padlock-closed.png]]		* [http://cshprotocols.cshlp.org/cgi/content/full/2006/3/pdb.rec8332?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=mops&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT&text_only=true CSH Protocols MOPS] [[Image:Padlock-closed.png]]
		+
		+	[[Category:Material]] and [[Category:Buffers]]

Stephen R. Bond: minor grammatical changes

2010-04-09T16:16:58Z

minor grammatical changes

←Older revision		Revision as of 16:16, 9 April 2010
Line 1:		Line 1:
	[[Image:800px-Mops2.JPG\|right\|200px\|thumb\|The other mops [http://en.wikipedia.org/wiki/Pug] ]]		[[Image:800px-Mops2.JPG\|right\|200px\|thumb\|The other mops [http://en.wikipedia.org/wiki/Pug] ]]

-	'''MOPS''' is the common name for the buffering compound in MOPS buffer. MOPS stands for ''3-(N-morpholino) propanesulfonic acid'' and with a pKa of 7.20, ~~MOPS is an~~ good ~~buffer~~ for many biological systems ~~at almost~~ neutral pH. [[HEPES]] is a chemically similar pH buffering compound.	+	'''MOPS''' is the common name for the buffering compound in MOPS buffer. MOPS stands for ''3-(N-morpholino) propanesulfonic acid'', and with a pKa of 7.20, makes a good buffering agent for many biological systems requiring neutral pH. [[HEPES]] is a chemically similar pH buffering compound.

	==Recipe for 10x MOPS buffer, 1 L==		==Recipe for 10x MOPS buffer, 1 L==
-	[[Image:800px-MOPS.png\|right\|200px\|thumb\|chemical structure of MOPS, 3-(N-morpholino) propanesulfonic acid]]	+	[[Image:800px-MOPS.png\|right\|200px\|thumb\|chemical structure of MOPS, 3-(N-morpholino) propanesulfonic acid]]

	* 83.7 g MOPS; MW 209.3 g/mol		* 83.7 g MOPS; MW 209.3 g/mol

Jakob Suckale at 18:23, 2 March 2009

2009-03-02T18:23:51Z

←Older revision		Revision as of 18:23, 2 March 2009
Line 3:		Line 3:
	'''MOPS''' is the common name for the buffering compound in MOPS buffer. MOPS stands for ''3-(N-morpholino) propanesulfonic acid'' and with a pKa of 7.20, MOPS is an good buffer for many biological systems at almost neutral pH. [[HEPES]] is a chemically similar pH buffering compound.		'''MOPS''' is the common name for the buffering compound in MOPS buffer. MOPS stands for ''3-(N-morpholino) propanesulfonic acid'' and with a pKa of 7.20, MOPS is an good buffer for many biological systems at almost neutral pH. [[HEPES]] is a chemically similar pH buffering compound.

-	==Recipe for 10x MOPS buffer==	+	==Recipe for 10x MOPS buffer, 1 L==
	[[Image:800px-MOPS.png\|right\|200px\|thumb\|chemical structure of MOPS, 3-(N-morpholino) propanesulfonic acid]]		[[Image:800px-MOPS.png\|right\|200px\|thumb\|chemical structure of MOPS, 3-(N-morpholino) propanesulfonic acid]]

Jakob Suckale at 18:22, 2 March 2009

2009-03-02T18:22:34Z

←Older revision		Revision as of 18:22, 2 March 2009
Line 18:		Line 18:
	* filter sterilise or autoclave		* filter sterilise or autoclave
	* store at room temperature		* store at room temperature
-	* protect from light; do not use if the solution ~~appears~~ yellow	+	* protect from light; do not use if the solution is dark (yellow is ok)

-	==Final concentration ~~of active compounds~~ in 10x stock==	+	==Final concentration in 10x stock==
	* 400 mM MOPS (buffering)		* 400 mM MOPS (buffering)
	* 100 mM NaAc		* 100 mM NaAc

Jakob Suckale: /* Stability of MOPS */

2009-03-02T18:20:42Z

Stability of MOPS

←Older revision		Revision as of 18:20, 2 March 2009
Line 32:		Line 32:

	==Stability of MOPS==		==Stability of MOPS==
-	Contrary to common belief, MOPS is sufficiently heat-stable to be autoclaved. Solution will turn yellow but this does not interfere with its buffering capacity. See, for example, Farrell RNA methods, p201 [http://books.google.de/books?id=h5lGFPz054oC&pg=PA201&dq=farrel+rna+mops&lr=&ei=RAGsSb_8NouYMp7enJIF&hl=en]. Straw coloured buffer is but do not use darker buffer.	+	Contrary to common belief, MOPS is sufficiently heat-stable to be autoclaved. Solution will turn yellow but this does not interfere with its buffering capacity. See, for example, Farrell RNA methods, p201 [http://books.google.de/books?id=h5lGFPz054oC&pg=PA201&dq=farrel+rna+mops&lr=&ei=RAGsSb_8NouYMp7enJIF&hl=en]. Straw coloured buffer is good but do not use darker buffer.

	==Some OWW protocols which use MOPS==		==Some OWW protocols which use MOPS==

Jakob Suckale at 18:20, 2 March 2009

2009-03-02T18:20:05Z

←Older revision		Revision as of 18:20, 2 March 2009
Line 1:		Line 1:
-	[[Image:800px-Mops2.JPG\|right\|200px\|thumb\|The other mops]]	+	[[Image:800px-Mops2.JPG\|right\|200px\|thumb\|The other mops [http://en.wikipedia.org/wiki/Pug] ]]

	'''MOPS''' is the common name for the buffering compound in MOPS buffer. MOPS stands for ''3-(N-morpholino) propanesulfonic acid'' and with a pKa of 7.20, MOPS is an good buffer for many biological systems at almost neutral pH. [[HEPES]] is a chemically similar pH buffering compound.		'''MOPS''' is the common name for the buffering compound in MOPS buffer. MOPS stands for ''3-(N-morpholino) propanesulfonic acid'' and with a pKa of 7.20, MOPS is an good buffer for many biological systems at almost neutral pH. [[HEPES]] is a chemically similar pH buffering compound.
Line 8:		Line 8:
	* 83.7 g MOPS; MW 209.3 g/mol		* 83.7 g MOPS; MW 209.3 g/mol
	* 13.6 g Sodium Acetate, trihydrate; MW 136.1 g/mol (watch out: NaAc, anhydrous is only 82 g/mol or 8.2g)		* 13.6 g Sodium Acetate, trihydrate; MW 136.1 g/mol (watch out: NaAc, anhydrous is only 82 g/mol or 8.2g)
-	* 2.9 g EDTA, ~~sodium salt~~; MW ~~292~~.2 g/mol	+	* 3.7 g EDTA, disodium dihydrate; MW 372.24 g/mol (again check MW of the salt you stock)


Line 29:		Line 29:
	* 200 mM MOPS - 41.9 g in 1 L		* 200 mM MOPS - 41.9 g in 1 L
	* 20 mM NaAc - 2.7 g		* 20 mM NaAc - 2.7 g
-	* 10 mM [[EDTA]] - 2.9 g	+	* 10 mM [[EDTA]] - 3.7 g

	==Stability of MOPS==		==Stability of MOPS==

Jakob Suckale: /* Recipe for 10x MOPS buffer */

2009-03-02T17:06:21Z

Recipe for 10x MOPS buffer

←Older revision		Revision as of 17:06, 2 March 2009
Line 7:		Line 7:

	* 83.7 g MOPS; MW 209.3 g/mol		* 83.7 g MOPS; MW 209.3 g/mol
-	* 13.6 g Sodium Acetate, trihydrate; MW 136.1 g/mol (watch out: NaAc, anhydrous is only 82 g/mol or 1.6g)	+	* 13.6 g Sodium Acetate, trihydrate; MW 136.1 g/mol (watch out: NaAc, anhydrous is only 82 g/mol or 8.2g)
	* 2.9 g EDTA, sodium salt; MW 292.2 g/mol		* 2.9 g EDTA, sodium salt; MW 292.2 g/mol

Jakob Suckale: split into 2 variations

2009-03-02T17:05:53Z

split into 2 variations

←Older revision		Revision as of 17:05, 2 March 2009
Line 6:		Line 6:
	[[Image:800px-MOPS.png\|right\|200px\|thumb\|chemical structure of MOPS, 3-(N-morpholino) propanesulfonic acid]]		[[Image:800px-MOPS.png\|right\|200px\|thumb\|chemical structure of MOPS, 3-(N-morpholino) propanesulfonic acid]]

-	* 41.9 g MOPS; MW 209.3 g/mol	+	* 83.7 g MOPS; MW 209.3 g/mol
-	* 2.7 g Sodium Acetate, trihydrate; MW 136.1 g/mol (watch out: NaAc, anhydrous is only 82 g/mol or 1.6g)	+	* 13.6 g Sodium Acetate, trihydrate; MW 136.1 g/mol (watch out: NaAc, anhydrous is only 82 g/mol or 1.6g)
	* 2.9 g EDTA, sodium salt; MW 292.2 g/mol		* 2.9 g EDTA, sodium salt; MW 292.2 g/mol

Line 21:		Line 21:

	==Final concentration of active compounds in 10x stock==		==Final concentration of active compounds in 10x stock==
-	* ~~200~~ mM MOPS (buffering)	+	* 400 mM MOPS (buffering)
-	* 20 mM NaAc	+	* 100 mM NaAc
	* 10 mM [[EDTA]] (nuclease inhibition by Mg2+ chelation)		* 10 mM [[EDTA]] (nuclease inhibition by Mg2+ chelation)

-	~~Note: Farrell uses higher concentrations for 10x stock: 400~~ mM MOPS~~, 100~~ mM NaAc, 10 mM EDTA	+	==Variation==
		+	Some protocols use a less concentrated MOPS buffer
		+	* 200 mM MOPS - 41.9 g in 1 L
		+	* 20 mM NaAc - 2.7 g
		+	* 10 mM [[EDTA]] - 2.9 g

	==Stability of MOPS==		==Stability of MOPS==

Jakob Suckale: /* External links */

2009-03-02T17:02:33Z

External links

←Older revision		Revision as of 17:02, 2 March 2009
Line 37:		Line 37:
	== External links ==		== External links ==
	* [http://en.wikipedia.org/wiki/MOPS Wikipedia MOPS]		* [http://en.wikipedia.org/wiki/MOPS Wikipedia MOPS]
		+	* [http://www.fermentas.com/techinfo/electrophoresis/rnaloading.htm#MOPS_ MOPS recipe at Fermentas]
		+	* [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=M5755\|SIGMA&N5=SEARCH_CONCAT_PNO\|BRAND_KEY&F=SPEC premade MOPS 10x from Sigma]
	* [http://cshprotocols.cshlp.org/cgi/content/full/2006/3/pdb.rec8332?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=mops&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT&text_only=true CSH Protocols MOPS] [[Image:Padlock-closed.png]]		* [http://cshprotocols.cshlp.org/cgi/content/full/2006/3/pdb.rec8332?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=mops&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT&text_only=true CSH Protocols MOPS] [[Image:Padlock-closed.png]]

Jakob Suckale at 16:54, 2 March 2009

2009-03-02T16:54:36Z

←Older revision		Revision as of 16:54, 2 March 2009
Line 2:		Line 2:

	'''MOPS''' is the common name for the buffering compound in MOPS buffer. MOPS stands for ''3-(N-morpholino) propanesulfonic acid'' and with a pKa of 7.20, MOPS is an good buffer for many biological systems at almost neutral pH. [[HEPES]] is a chemically similar pH buffering compound.		'''MOPS''' is the common name for the buffering compound in MOPS buffer. MOPS stands for ''3-(N-morpholino) propanesulfonic acid'' and with a pKa of 7.20, MOPS is an good buffer for many biological systems at almost neutral pH. [[HEPES]] is a chemically similar pH buffering compound.
-

	==Recipe for 10x MOPS buffer==		==Recipe for 10x MOPS buffer==
-
	[[Image:800px-MOPS.png\|right\|200px\|thumb\|chemical structure of MOPS, 3-(N-morpholino) propanesulfonic acid]]		[[Image:800px-MOPS.png\|right\|200px\|thumb\|chemical structure of MOPS, 3-(N-morpholino) propanesulfonic acid]]

Line 21:		Line 19:
	* store at room temperature		* store at room temperature
	* protect from light; do not use if the solution appears yellow		* protect from light; do not use if the solution appears yellow
-

	==Final concentration of active compounds in 10x stock==		==Final concentration of active compounds in 10x stock==
Line 29:		Line 26:

	Note: Farrell uses higher concentrations for 10x stock: 400 mM MOPS, 100 mM NaAc, 10 mM EDTA		Note: Farrell uses higher concentrations for 10x stock: 400 mM MOPS, 100 mM NaAc, 10 mM EDTA
-

	==Stability of MOPS==		==Stability of MOPS==
Line 38:		Line 34:
	* [[Knight:NuPAGE electrophoresis]]		* [[Knight:NuPAGE electrophoresis]]
	* [[Sauer:bis-Tris SDS-PAGE, the very best]]		* [[Sauer:bis-Tris SDS-PAGE, the very best]]
-

	== External links ==		== External links ==
	* [http://en.wikipedia.org/wiki/MOPS Wikipedia MOPS]		* [http://en.wikipedia.org/wiki/MOPS Wikipedia MOPS]
	* [http://cshprotocols.cshlp.org/cgi/content/full/2006/3/pdb.rec8332?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=mops&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT&text_only=true CSH Protocols MOPS] [[Image:Padlock-closed.png]]		* [http://cshprotocols.cshlp.org/cgi/content/full/2006/3/pdb.rec8332?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=mops&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT&text_only=true CSH Protocols MOPS] [[Image:Padlock-closed.png]]