MRNA stability: Difference between revisions
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These data are consistent with measured half-lives for endogenous ''E. coli'' mRNA <cite>Bernstein</cite>. Bernstein ''et al.'' report that 80% of ''E. coli'' mRNA half-lives are between 3 and 8 min. Bernstein ''et al'' measured a global average mRNA half-life for ''E. coli'' of 5.7 min. | These data are consistent with measured half-lives for endogenous ''E. coli'' mRNA <cite>Bernstein</cite>. Bernstein ''et al.'' report that 80% of ''E. coli'' mRNA half-lives are between 3 and 8 min. Bernstein ''et al'' measured a global average mRNA half-life for ''E. coli'' of 5.7 min. | ||
==References== | |||
<biblio> | <biblio> | ||
#Bernstein pmid=12119387 | #Bernstein pmid=12119387 | ||
</biblio> | </biblio> | ||
Revision as of 09:34, 3 September 2007
What is the half-life of a typical mRNA in E. coli?
Jennifer Braff and Caitlin Conboy in the Endy Lab at MIT measured the half-lives of some transcripts in E. coli MC4100 in a chemostat. This work was part of the Soup to nuts project.
They measured mRNA half-lives for the following BioBrick parts -
| Construct | mRNA Half-life
(min) |
|---|---|
| pSB4A3-I7100 | 3.13±0.48 |
| pSB3K3-I7100 | 5.36±2.07 |
| pSB4A3-I7101 | 2.19±0.28 |
| pSB3K3-I7101 | 2.24±0.33 |
| pSB4A3-I7107 | 0.98±0.04 |
| pSB3K3-I7107 | 2.18±0.34 |
| pSB4A3-I7109 | 1.55±0.18 |
| pSB3K3-I7109 | 3.08±0.65 |
These data are consistent with measured half-lives for endogenous E. coli mRNA [1]. Bernstein et al. report that 80% of E. coli mRNA half-lives are between 3 and 8 min. Bernstein et al measured a global average mRNA half-life for E. coli of 5.7 min.
