What is the half-life of a typical mRNA in E. coli?
They measured mRNA half-lives for the following BioBrick parts -
|mRNA degradation rate
From the above data, we can calculate a mean value of 4.8E-3 for the three devices using B0032 on pSB3k3 (I7101, I7107, and I7109).
These data are consistent with measured half-lives for endogenous E. coli mRNA . Bernstein et al. report that 80% of E. coli mRNA half-lives are between 3 and 8 min. Bernstein et al. measured a global average mRNA half-life for E. coli of 5.7 min.
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