Macrophage therapy

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==Project Details and Methods==
==Project Details and Methods==
 +
* Methods used by the Haney Paper:
 +
** standard cloning with luciferase and gfp reporters
 +
** transfection
 +
** mouse macrophage cells, Cath.A neurons
 +
** Balb mice
 +
**
==Possible Project Outcomes==
==Possible Project Outcomes==
==Resources Needed==
==Resources Needed==

Revision as of 17:30, 6 May 2013

Contents

Project Overview

This page is for a 20.109 project by Katie and Emily.


Background Information

  • pathology of Alzheimers and Parkinsons Disease
    • activation and secretion of reactive oxygen and nitrogen species, leads to oxidative stress, affects function of neurons, astrocytes, and microglia
      • induces ion transport, calcium mobilization, apoptotic programs
    • mitochondrial respiratory chain affects oxidative phosphoyrlation and is responsible for the production of Reactive Oxygen Species
    • observe lack of natural antioxidants such as glutathione and superoxide dismutase, and iron in the SN region of the brain
  • a way to cure these diseases:
    • find a way to deliver redox enzymes to the brain
    • the paper below takes advantage of macrophages since they can carry the antioxidant proteins across the blood brain barrier - to specifically target subregions of the brain


  • include comparison of "gold standard" for gene therapy techniques?
  • find other sources citing macrophages as delivery vectors?
  • The reduction of brain inflammation is important in the treatment of neurodegenerative diseases. The study below used genetically modified macrophages, carrying reporters and therapeutic genes, in order to upregulate catalase, an enzyme that reduces inflammation. They particularly targeted Parkinson’s disease in mice, and noted a three fold reduction in brain inflammation and a significant improvement in motor functions. This suggests that macrophages have potential as vectors for gene and drug delivery for these types of disorders.

Haney, M et al. (2013), Specific transfection of inflamed brain by macrophages: a new therapeutic strategy for neurodegenerative diseases. PNAS, 8(4).

Research Problems and Goals

  • Challenges with targeting neurodegenerative diseases:
    • limited blood brain barrier permeability
    • inherent peripheral and brain drug permeabilities
    • low therapeutic indices

Project Details and Methods

  • Methods used by the Haney Paper:
    • standard cloning with luciferase and gfp reporters
    • transfection
    • mouse macrophage cells, Cath.A neurons
    • Balb mice

Possible Project Outcomes

Resources Needed

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