Madhadron:ChromosomeMarker: Difference between revisions
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Revision as of 08:40, 27 November 2006
This protocol is a port of the system described in Nielsen, Ottesen, etc. (2006) in Molecular Microbiology to mycobacteria.
Background
Two component system from phage: parS is a binding site for the ParB protein. parS is inserted in the chromosome (a single copy appears to be sufficient). ParB is expressed inducibly as a fusion protein with a GFP on a plasmid.
Two organisms already adapted thus: P1 phage, and pMT phage (from Y. pestis; uses a deletion of the the 23 N-terminal amino acids of ParB instead of the whole thing).
