Magni:Back Door: Difference between revisions
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(New page: {{Magni}} <div style="padding: 10px; width: 720px; border: 5px solid #000000;"> == General == [http://www.labmedinfo.org/] </div>) |
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== | == Antibiotics, inducers and substrates == | ||
*Ampicillin - 100 mg/ml in deionized water (-20°C) | |||
*Chloramphenicol - 34 mg/ml in ethanol (-20°C) | |||
*Kanamycin - 50 mg/ml in deionized water (-20°C) | |||
*Gentamycin - 50 mg/ml in deionized water (-20°C) | |||
*G418 - 50 mg/ml in deionized water (+4°C) | |||
*HSL (N-3-oxo-C6-L-homoserine lactone) - 2 mM in water (-20°C) | |||
*aTc (Anhydrotetracycline) - 2 mg/ml in ethanol or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE | |||
*IPTG Ready Made - 200 mM (-20°C) | |||
*X-Gal - 40 mg/ml in DMF (-20°C) | |||
== ''E. coli'' strains == | |||
*BW20767 (Registry) | |||
*BW23474 (CGSC) | |||
*BW25141 (CGSC) | |||
*BW25142 (CGSC) | |||
*CW2553 + pJat8 (Registry) | |||
*DB3.1 (Invitrogen) | |||
*DH5alpha (Invitrogen) | |||
*KRX (Promega) | |||
*MC1061 (CGSC) | |||
*MG1655 (CGSC) | |||
*MG1655-Z1 (Univ of Washington) | |||
*TOP10 (Invitrogen) | |||
*W3110 (DSMZ) | |||
*XL1-Blue (Stratagene) | |||
</div> | </div> | ||
Revision as of 10:40, 28 March 2013
Antibiotics, inducers and substrates
- Ampicillin - 100 mg/ml in deionized water (-20°C)
- Chloramphenicol - 34 mg/ml in ethanol (-20°C)
- Kanamycin - 50 mg/ml in deionized water (-20°C)
- Gentamycin - 50 mg/ml in deionized water (-20°C)
- G418 - 50 mg/ml in deionized water (+4°C)
- HSL (N-3-oxo-C6-L-homoserine lactone) - 2 mM in water (-20°C)
- aTc (Anhydrotetracycline) - 2 mg/ml in ethanol or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE
- IPTG Ready Made - 200 mM (-20°C)
- X-Gal - 40 mg/ml in DMF (-20°C)
E. coli strains
- BW20767 (Registry)
- BW23474 (CGSC)
- BW25141 (CGSC)
- BW25142 (CGSC)
- CW2553 + pJat8 (Registry)
- DB3.1 (Invitrogen)
- DH5alpha (Invitrogen)
- KRX (Promega)
- MC1061 (CGSC)
- MG1655 (CGSC)
- MG1655-Z1 (Univ of Washington)
- TOP10 (Invitrogen)
- W3110 (DSMZ)
- XL1-Blue (Stratagene)
