Magni:Back Door

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(General)
Current revision (13:40, 28 March 2013) (view source)
(E. coli strains)
 
(11 intermediate revisions not shown.)
Line 3: Line 3:
<div style="padding: 10px; width: 720px; border: 5px solid #000000;">
<div style="padding: 10px; width: 720px; border: 5px solid #000000;">
-
== General ==
+
== Antibiotics, inducers and substrates ==
-
http://www.labmedinfo.org/
+
*Ampicillin - 100 mg/ml in deionized water (-20°C)
 +
*Chloramphenicol - 34 mg/ml in ethanol (-20°C)
 +
*Kanamycin - 50 mg/ml in deionized water (-20°C)
 +
*Gentamycin - 50 mg/ml in deionized water (-20°C)
 +
*G418 - 50 mg/ml in deionized water (+4°C)
 +
*HSL (N-3-oxo-C6-L-homoserine lactone) - 2 mM in water (-20°C)
 +
*aTc (Anhydrotetracycline) - 2 mg/ml in ethanol or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE
 +
*IPTG Ready Made - 200 mM (-20°C)
 +
*X-Gal - 40 mg/ml in DMF (-20°C)
 +
 
 +
== ''E. coli'' strains ==
 +
*BW20767 (Registry)
 +
*BW23474 (CGSC)
 +
*BW25141 (CGSC)
 +
*BW25142 (CGSC)
 +
*CW2553 + pJat8 (Registry)
 +
*DB3.1 (Invitrogen)
 +
*DH5alpha (Invitrogen)
 +
*KRX (Promega)
 +
*MC1061 (CGSC)
 +
*MG1655 (CGSC)
 +
*MG1655-Z1 (Univ of Washington)
 +
*TOP10 (Invitrogen)
 +
*W3110 (DSMZ)
 +
*XL1-Blue (Stratagene)
-
http://cit.unipv.it/cit/
 
</div>
</div>

Current revision

Home        Contact        Lab Members        Publications        Research        Internal        Protocols        Data       


Antibiotics, inducers and substrates

  • Ampicillin - 100 mg/ml in deionized water (-20°C)
  • Chloramphenicol - 34 mg/ml in ethanol (-20°C)
  • Kanamycin - 50 mg/ml in deionized water (-20°C)
  • Gentamycin - 50 mg/ml in deionized water (-20°C)
  • G418 - 50 mg/ml in deionized water (+4°C)
  • HSL (N-3-oxo-C6-L-homoserine lactone) - 2 mM in water (-20°C)
  • aTc (Anhydrotetracycline) - 2 mg/ml in ethanol or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE
  • IPTG Ready Made - 200 mM (-20°C)
  • X-Gal - 40 mg/ml in DMF (-20°C)

E. coli strains

  • BW20767 (Registry)
  • BW23474 (CGSC)
  • BW25141 (CGSC)
  • BW25142 (CGSC)
  • CW2553 + pJat8 (Registry)
  • DB3.1 (Invitrogen)
  • DH5alpha (Invitrogen)
  • KRX (Promega)
  • MC1061 (CGSC)
  • MG1655 (CGSC)
  • MG1655-Z1 (Univ of Washington)
  • TOP10 (Invitrogen)
  • W3110 (DSMZ)
  • XL1-Blue (Stratagene)
Personal tools